Objective:To establish the experimental nude mouse model of endometriosis for human eutopic endometrium.Methods:Female nude mouse aged 6~8 weeks were selected.Nude mouse in experimental group(n=20)were implanted with the endometrium of patients with endometriosis or without endometriosis into pelvic and abdominal cavities.Nude mouse in control group(n=3)were implanted with the greater omentum.Nude mouse were killed at 5th

15th

and 30th day after implantation for observ ation of the growth of endometriotic lesions.The morphological and histological changes of endometriotic lesions from diferent sources and at diferent time points of growth were examined by light microscopy.Results:The achievement ratio of transplantation was 100%.At the 5th day

endometriotic lesions were found in the pelvic and abdominal cavities

and the adhesion to mice tissues was already tight.There were nascent vascular net between endometriotic lesions and mice mesepithelium.Endometrial glands around with stroma cells were found under light microscope.At the 15th day

endometriotic lesions mixed with mice tissues.Endometriotic lesions presented parenchymatous and cystiform.A lot of endometrial glands and stroma cells with rich blood supply were seen under light microscope.At the 30th day

endometriotic lesions became atrophied

and the adhension was serve partly.Endometriotic lesions presented severe fibrosis and some endometrial cells were not in integrity under light microscope.In the control group

some of the greater omentum adhered to the incisal opening of abdominal wall

while the others were free and atrophied or merged gradually.There was no change in the constructed greater omentum except for some inflammatory reaction.Conclusions:Nude mouse model of endometriosis was good for studying the endometria cell proliferation

neovascularization

hormone

immune response and so on

since the implanted endometrium remain the original morphological and histological features.It is a perfect model of endometriosis because of its significant and steady character

low cost

simple operation and short observation cycle.