Objectives:To evaluate the effects of PEDF eyedrops on the corneal allograft rejection by topical installition in the rats model of penetrating keratoplasty

and to investigate its possible mechansism and its correlation factor.Methods:Penetrating keratoplasty(PKP)was proformed orthotopically in wistar rats and SD rat recipients.60 SD rats were randomly assigned to two groups;experimental group and experimental control group after PKP.30 SD rats underwent auto penetrating keratoplasty(blank control group).The experimental group was treated with PEDF eyedrops

while the blank control group and experimental control group were treated with escipient eyedrops.All animals’surrival time was recored and compared by the biomicroscope examination.6 animals in three groups were killed respectively at the 1st week

2nd week

3rd week

4th week and 5th week postoperatively.Corneal ultrastuctural morphology was observed by TEM.PEDF and VEGF expression was examined by immunohistochemistry.Results:Expression level of VEGF of experimental control group was higher than that of experimental group(P<0.05 or P<0.01).Conclusion:PEDF eyedrops can depress expression of VEGF in corneal allografts.Topical application of PEDF eyedrops can prolong suivival time of the allografts.