Objective:To investigate the effects of salidroside on the proliferation

activation

Collagen Ⅰ secretion as well as expression of ROCK-Ⅰand TIMP-1 in HSC-T6 cell

and to elucidate the molecular mechanism of salidroside against hepatic fibrosis with Y-27632 as the positive control. Methods: HSC-T6 cell line was employed as the model for following study. MTT colorimetric assay was used to detect the proliferation of HSC-T6 cell. ELISA was employed to value the content of collagen Ⅰ in supernatant of HSC-T6 cells treated with salidroside and Y-27632. The effects of salidroside and Y-27632 on expressions of smooth muscle actin (α-SMA)、ROCK-Ⅰand TIMP-1 expression were analyzed by Real-time PCR and Western blot. Results(:1)Both Salidroside(3、6、12 mmol/L)and Y-27632(5、 10、20 μmol/L)had dose-dependent inhibitory effects on α-SMA expression which represents the activated state of HSC-T6(P<0.05) (2)The expression ofROCK-I could be downregulated bythe 6 mmol/Lsalidroside and 10 μmol/LY-27632 in HSC-T6(P<0.05).(3) The 6mmol/L salidroside and 10 μmol/L Y-27632 both had inhibitory effects on TIMP-1 expression and collagen Ⅰ output in HSC-T6 cell(P<0.05). Conclusion:Salidroside can inhibit activation

TIMP-1 expression and collagen Ⅰ secretion of HSC-T6 cell

the mecha-nisms ofwhich maybe involved with its inhibitoryeffect on Rho/ROCKsignal transduction pathway.