Objective:To investigate the changes of intestinal flora and the effects of antibiotics and micro-ecological agent on the intestinal flora in the elderly diabetes.Methods:Intestinal flora detection by Stool cultures

compared 55 diabetes with 184 non-diabetes.Those who need antibiotics were randomly divided into two groups:one group used micro-ecological agent(bacillus licheniformis capsules) and the other not.Their fecal were collected to detect intestinal flora before antibiotic used and at the 5th、10th and 15th day of antibiotic used.Results:Compared with non-diabetes

the quantity of Escherichia coli significantly increased(9.53±1.76 vs 9.14±1.29

P<0.05) and the quantities of Bifidobacterium(7.02±1.85 vs 8.31±2.17

P<0.05)and Lactobacillus(6.75±2.86 vs 7.51±2.36

P<0.05)significantly decreased in the elderly diabetes.In antibiotic using process

the quantities of Bifidobacterum

Lactobacillum and Bacteroides decreased gradually

especially significant at the 10th and 15th day of antibiotic used.Compared with those who did not use micro-ecological agent

the quantities of Bifidobacterum

Lactobacillum and Bacteroides in those who used micro-ecological agent increased to some degrees.There was a positive correlation between Bifidobacterum’s quantity and age(r=0.358

P<0.05).There was a negative correlation between Bifidobacterum’s quantity and glycosylated hemoglobin(r=-0.479

P<0.05).Conclusion:There were maladjustment of intestinal flora in the elderly diabetes

the quantities of Bifidobacterium and Lactobacillus decreased and Escherichia coli’ increased.There were relationship between poor glycemic control and the decreasing of bifidobacterum.Long-term using antibiotics made bifidobacterum and Lactobacillum’s quantities decreased.Using micro-ecological agent could Suppress alteration of intestinal flora caused by antibiotics.