Objective:To investigate the inclusion process and taste masking effect of hydroxypropyl cyclodextrin(HP-β-CD)on the extract of Gentian.Method:The inclusion complex was prepared by grinding method.The multiple factors and levels experiments were done by orthogonal experiments design with the bitterness score as criteria.Then the optimum preparation was established.Results:The optimum entrapment conditions were as follows:the concentration of HP-β-CD was 20%;the grinding time was 5min

the bath temperature was 55℃;and the bath time was 6h.The formation of inclusion complex of the Gentian extract was confirmed by the change of differential scanning calorimetry and ultraviolet spectrophotometry.Conclusion:The inclusion compound from the extract of gentian cyclodextrin was prepared.The inclusion complex showed significant effects of bitter taste masking.