Survivin基因及其主要剪接变构体mRNA在大肠癌组织中的表达

左文革; 李瑜元; 聂玉强; 沙卫红; 王红; 林泳

您当前的位置：

首页 >

文章列表页 >

Survivin基因及其主要剪接变构体mRNA在大肠癌组织中的表达

肿瘤分子学相关研究 | 更新时间：2024-01-17

- Survivin基因及其主要剪接变构体mRNA在大肠癌组织中的表达
- Expression of survivin gene and main transcript variants mRNA in colorectal carcinomas
- 重庆医科大学学报 2009年34卷第7期页码：852-856
- 作者机构：
  
  广州医学院附属广州市第一人民医院消化内科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R735.34
- 纸质出版日期：2009，
扫描看全文
[1]左文革,李瑜元,聂玉强,沙卫红,王红,林泳.Survivin基因及其主要剪接变构体mRNA在大肠癌组织中的表达[J].重庆医科大学学报,2009,34(07):852-856.

ZUO Wen-ge, et al. Expression of survivin gene and main transcript variants mRNA in colorectal carcinomas[J]. Journal of Chongqing Medical University, 2009,34(7):852-856.
[1]左文革,李瑜元,聂玉强,沙卫红,王红,林泳.Survivin基因及其主要剪接变构体mRNA在大肠癌组织中的表达[J].重庆医科大学学报,2009,34(07):852-856. DOI：

ZUO Wen-ge, et al. Expression of survivin gene and main transcript variants mRNA in colorectal carcinomas[J]. Journal of Chongqing Medical University, 2009,34(7):852-856. DOI：

摘要

目的:探讨大肠癌组织中Survivin基因及其主要剪接变构体与癌胚抗原（Carcinoembryonic antigen

CEA）CAM5mRNA表达的临床意义。方法:39例大肠癌切除标本

采集肿瘤组织、癌旁2.5cm组织和距离肿瘤10cm以上的正常大肠组织各1块

采用RT-PCR方法检测总survivin、survivin-△Ex3、survivin-2B、survivin-3B、survivin2α和CEACAM5 mRNA的表达。用凝胶图像分析系统或SYBRGreenⅠ实时荧光定量PCR检测上述基因mRNA的表达水平。结果:总survivin、survivin-△Ex3、survivin-2B、sur-vivin-3B、survivin2α和CEACAM5mRNA在大肠癌组织中的阳性表达率分别为100.0%、92.3%、92.3%、79.5%、87.2%和97.4%

均高于癌旁2.5cm组织中的38.5%、25.6%、28.2%、7.7%、12.8%和12.8%

差异均有统计学意义（P<0.01）。正常肠壁组织中上述mRNA的阳性表达率分别为17.9%、5.1%、7.7%、0.0%、2.6%和10.3%

与癌旁2.5cm组织比较

总survivin、survivin-△Ex3和sur-vivin-2B差异有统计学意义（P<0.05）。总survivin及其剪接变构体和CEACAM5mRNA的表达水平

大肠癌组织高于癌旁2.5cm组织（P<0.01）;癌旁2.5cm组织与正常组织的差异均无统计学意义（P>0.05）。survivin剪接变构体mRNA的表达水平与一些临床病理参数有关（P<0.05）。结论:Survivin和其剪接变构体mRNA在大肠癌组织中呈高表达

表达水平与恶性临床病理参数有关。检测Survivin和其剪接变构体mRNA表达对大肠癌前预报与早期诊断比CEAmRNA更有价值。

Abstract

Objective: To investigate the clinical significance of the expressions of survivin gene and its main transcript variants mRNA in colorectal carcinoma. Methods: The expression of total survivin

survivin-△Ex3

survivin-2B

survivin-3B

survivin 2α

and CEA CAM 5 mRNA in 39 cases colorecta1 cancer

adjacent tissues (2.5 cm from the cancer margin) and norma1 colorecta1 tissue (10 cm from the cancer margin) were detected by RT-PCR technique and semi-quantitative methods use gel imaging analytical system or SYBR GreenⅠreal-time fluorescent PCR. Results: The positive rates of total survivin

survivin-△Ex3

survivin-2B

survivin-3B

survivin2α

and CEA CAM5 mRNA in colorectal carcinoma and adjacent tissue were 100.0%

92.3%

79.5%

87.2%

97.4%

and 38.5%

25.6%

28.2%

7.7%

12.8%

showing significant difference in two tissues (P<0.01);The positive rates were 17.9%

5.1%

7.7%

0.0%

2.6% and 10.3% in norma1 colorecta1 tissues

respectively

showing significant difference in total survivin

survivin-△Ex3 and survivin-2B from adjacent tissues (P<0.05). Total survivin

four transcript variants and CEA CAM5 mRNA levels were higher in colorectal carcinoma than in adjacent tissue (P<0.01);but no significant difference was found between adjacent carcinoma and norma1 colorecta1 tissues (P>0.05). Several transcript variants mRNA levels were associated with some clinicopathologic parameters (P<0.05). Conclusion: The survivin and its transcript variants mRNA were highly expressed in colorectal carcinomas

several transcript variants mRNA levels were associated with clinicopathologic parameters. Detection of Survivin transcript variants mRNA expression may be more valuable than the CEA mRNA in early prediction of colorectal carcinoma.

关键词

Survivin剪接变构体癌胚抗原CAM5大肠癌表达凋亡

Keywords

Survivin transcript variantCarcinoembryomic antigen (CAM5)Colorectal carcinomaexpressionapoptosis

references

浏览量

102

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

LncRNA MYLK-AS1调节miR-141-3p/STMN1轴对胃癌细胞增殖、凋亡和侵袭的影响

TNF-α下调大鼠肺微血管内皮细胞紧密连接蛋白ZO-1、Claudin-5的表达

敲低RBX1通过下调PHF5A抑制胰腺癌细胞增殖，迁移和侵袭并促进凋亡

XL413通过抑制DNA复制诱导细胞凋亡来抑制结肠癌细胞增殖

3-甲基腺嘌呤通过抑制自噬和内质网应激途径保护睡眠紊乱诱导的大鼠肝损伤