不同剂量拉莫三嗪对体外培养海马神经细胞存活及其BDNF和P-CREB表达的影响

李佩瑾; 蒋莉; 刘官信

您当前的位置：

首页 >

文章列表页 >

不同剂量拉莫三嗪对体外培养海马神经细胞存活及其BDNF和P-CREB表达的影响

基础研究 | 更新时间：2024-01-17

- 不同剂量拉莫三嗪对体外培养海马神经细胞存活及其BDNF和P-CREB表达的影响
- Effect of lamotrigine of different concentration on the survival rate and the expression of BDNF and p-CREB in the cultured hippocampus neuron
- 重庆医科大学学报 2009年34卷第10期页码：1330-1333
- 作者机构：
  
  重庆医科大学附属儿童医院神经内科
- 作者简介：
- 基金信息：
- DOI：
  中图分类号： R742.1
- 纸质出版日期：2009，
扫描看全文
[1]李佩瑾,蒋莉,刘官信.不同剂量拉莫三嗪对体外培养海马神经细胞存活及其BDNF和P-CREB表达的影响[J].重庆医科大学学报,2009,34(10):1330-1333.

LI Pei-jin, et al. Effect of lamotrigine of different concentration on the survival rate and the expression of BDNF and p-CREB in the cultured hippocampus neuron[J]. Journal of Chongqing Medical University, 2009,34(10):1330-1333.
[1]李佩瑾,蒋莉,刘官信.不同剂量拉莫三嗪对体外培养海马神经细胞存活及其BDNF和P-CREB表达的影响[J].重庆医科大学学报,2009,34(10):1330-1333. DOI：

LI Pei-jin, et al. Effect of lamotrigine of different concentration on the survival rate and the expression of BDNF and p-CREB in the cultured hippocampus neuron[J]. Journal of Chongqing Medical University, 2009,34(10):1330-1333. DOI：

摘要

目的:观察不同浓度拉莫三嗪（Lamotrigine

LTG）对体外培养海马神经细胞存活影响及脑源性神经生长因子（Brainderived neurotrophic factor

BDNF）和磷酸化cAMP反应元件结合（Phosphorylated cAMP response element binding protein

p-CREB）的表达变化。从细胞水平角度探索拉莫三嗪对认知功能的可能影响。方法:取培养8 d的成熟海马神经细胞

随机分为拉莫三嗪低剂量组（4 mg/L）、拉莫三嗪中剂量组（8 mg/L）、拉莫三嗪高剂量处理组（12 mg/L）和空白对照组（0.1%DMSO）。维持浓度培养4 d后

MTT法检测细胞存活数量

免疫组化检测神经细胞BDNF和p-CREB。结果:（1）不同浓度拉莫三嗪处理组与空白对照组之间比较

细胞存活率有明显差异（P<0.05）。而3个不同剂量拉莫三嗪组之间的细胞存活率没有显著差异（P>0.05）。（2）与正常对照组比12 mg/L拉莫三嗪培养神经细胞

p-CREB和BDNF的表达显著降低（P<0.05）

而4、8 mg/L拉莫三嗪剂量组无差异（P>0.05）。（3）加入拉莫三嗪12 mg/L培养神经细胞BDNF和p-CREB的表达明显低于4、8 mg/L拉莫三嗪剂量组（P<0.05）。结论:在4～12 mg/L的剂量范围内

拉莫三嗪均可造成体外培养神经细胞死亡

高浓度拉莫三嗪可通过神经细胞内BDNF和p-CREB的表达变化

影响神经细胞存活。

Abstract

Objective:To explore the possible effections on neuron survival rate and the change of BDNF and p-CREB induced by different concentrations of lamotrigine.Methods: The hippocampus was harvested from newborn Sprague-Dawley rats.Primary hippocampal neuron cultured for 8 days in vitro were used and divided randomly into four groups: low concentration(4 mg/L) group

middle concentration(12 mg/L) group

high concentration(12 mg/L) group and 0.1%DMSO as control.The BDNF and p-CREB levels were determined by immunohistochemistry and the cell survival rate was tested by MTT.Results:(1)compared with that in the control group

the survival of the neuron cell in all the lamotrigine groups decreased significantly(P<0.05)

but the differences of survival rate was not great among the three lamotrigine groups.(2) Compared with the control group

expression of BDNF and p-CREB on the cell in the high concentration group were significantly different from those in the control group(P<0.05).(3) BDNF and p-CREB expressing on the cell in the 12 mg/L lamotrigine concentration group were significantly different from those in the 4 mg/L and 8 mg/L lamotrigine concentration group(P<0.05).Conclusion: In the lever of 4~12 mg/L

lamtrigine can cause the neural cell death in vitro and the high concentration of lamotrigine(12 mg/L) causes the cell death by changing the expression of BDNF and p-CREB

关键词

拉莫三嗪p-CREB认知

Keywords

Lamotriginep-CREBRecognition

references

浏览量

182

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

暂无数据