Objective:To screen and optimize the lung cancer related tumor markers from multi-tumor marker protein chip.Methods:With the multi-tumor marker protein chip

the sera of 311 patients with lung cancer were detected for multi-tumor markers including carcinoembryonic antigen(CEA)

alpha fetoprotein(AFP)

carbohydrate antigen 199(CA199)

cancer antigen 153(CA153)

cancer antigen 125(CA125)

prostate specific antigen(PSA)

free-PSA

and ferritin.Lung cancer related parameters were analyzed by Kappa test and cost-effectiveness analysis.Results:There were four kinds of tumor markers which were apparently increased in the serum among the 311 lung cancer patients:CEA(41.5%)

CA153(15.2%)

CA199(20.9%)

and CA125(37.0%).Five tumor marker combinations were found to be strongly consistent with the results of multi-tumor marker protein chip by Kappa test

and the combination of CEA with CA125 was proved to be the best by cost-effectiveness analysis.Conclusion:The combined detection of sera CEA and CA125 is helpful to diagnose lung cancer

which is consistent with the positive rate of diagnosis by using multi-tumor marker protein chip and the two combination test can cut down the cost of diagnosis of lung cancer.