Objective:To investigate the effect of tumor necrosis factor-α(TNF-α) intervention on the expression and secretion of pigment epithelium-derived factor(PEDF) in 3T3-L1 adipocytes.Methods:3T3-L1 preadipocytes were cultured and differentiated into the mature adipocyte.3T3-L1 adipocytes were incubated with TNF-α at different concentrations(0.1

1

10

100 ng/ml) for different periods of time(3

6

12

24 h).PEDF mRNA expression was detected by Real-time Quantitative PCR.Total protein of PEDF secreted by these adipocytes was evaluated by ELISA.Results:(1)Treatment of 3T3-L1 adipocytes with TNF-α at different concentrations decreased PEDF mRNA expression and secretion along with the elevated TNF-α concentration in the culture medium(P<0.05).(2)The inhibitive effect of TNF-α(10 ng/ml) on PEDF mRNA expression gradually strengthened with the increase of treatment time

and reached the maximum at 12 h(P<0.05).(3)Treated with TNF-α(10 ng/ml)

the inhibition rate of their secretion of PEDF was 15% at 6 h

and 33% at 24 h(P<0.05).Conclusion:TNF-α significantly decreases the expression and secretion of PEDF in 3T3-L1 adipocytes in a dose-and time-dependent manner.