Objective：To discuss the effect of the chitosan thermosensitive hydrogel loading Drynaria Rhizome，Coptis chinensis and Panax notoginseng（sanhuangbu） on the repair of periodontal defects. Methods：Totally 36 male SD rats were enrolled and periodon－tal fenestration model in mandibular molar area was prepared. Then all rats were divided into three groups in matched pair：group filled with chitosan thermosensitive hydrogel loading sanhuangbu（hydrogel and medicine group），group filled with nothing or filled only with chitosan thermosensitive hydrogel（blank hydrogel group）and blank control group. In each group，animals were sacrificed on 14 d（n=18） and 28 d（n=18） postoperatively and the mandibles were processed for histological examination by HE staining. Results：Area of new alveolar bone was extremely lager in hydrogel and medicine group than in blank hydrogel group and blank control group on 14 d and 28 d postoperatively with statistical differences（P=0.000）. Area of new alveolar bone was extremely lager in blank hydrogel group than in blank control group with statistical differences（P=0.000）. Conclusion：Chitosan thermosensitive hydrogel loading sanhuangbu can effectively promote the periodonontal tissue regeneration.