Objective：To observe the carrier status and drug resistance of group B streptococci（GBS） in perinatal pregnant women and their newborns and to improve patients’ life quality. Methods：Totally 426 perinatal pregnant women and 428 newborns were selected in our hospital and were received GBS bacteria identification and drug sensitivity test. Results were recorded and analyzed. Results：Positive rate of 426 cases of perinatal pregnant GBS was 19.01%，significantly higher than that of newborn（1.40%），with statistically significant differences（ ?字2=72.38，P=0.000）. GBS showed 100% sensitivity to ampicillin and penicillin. Drugs mentioned above can be used in clinics to achieve satisfactory effects. Conclusions：GBS test should be employed in the clinical work. If infection confirmed，the active therapeutic measures should be tak－en to avoid mother-to-child transmission，premature delivery，late abortion and to improve the perinatal maternal and neonatal life quality.