Objective：To evaluate the differential diagnosis of 3.0T dynamic contrast-enhanced magnetic resonance imaging（DCE-MRI） combined with diffusion weighted imaging（DWI） in the characterization of breast lesion. Methods：A total of 73 patients with breast diseases（37 benign lesions and 42 malignant lesions） were retrospectively analyzed. All lesions were examined with 3.0T DCE-MRI and DWI（b=0.800 s/mm2） before the histopathological demonstration. The lesion morphology，edge enhancement and curve pattern were analyzed using integral method and statistical method. The Fisher score，sensitivity，specificity，accuracy，positive likelihood ra－tio，and negative likelihood ratio of DWI and combined method in the diagnosis of breast lesions were compared. Results：The average apparent diffusion coefficient（ADC） of benign and malignant lesions were（1.49±0.34）×10-3 mm2/s and（1.01±0.29）×10-3 mm2/s.The threshold of ADC between benign and malignant lesions was 1.15×10-3 mm2/s by receiver operating characteristic curve. The sen－sitivity，specificity，accuracy and positive likelihood ratio，negative likelihood ratio of ADC were 83.3%，83.8%，83.5%，0.199，5.142.The sensitivity，specificity，accuracy and positive likelihood ratio，negative likelihood ratio of DCE-MRI were 97.6%，78.4%，88.6%，0.031，4.519. The sensitivity，specificity，accuracy，positive likelihood ratio and negative likelihood ratio of DCE-MRI combined with DWI were92.9%，91.9%，92.4%，0.077，11.469. Conclusion：DCE-MRI combined with DWI is of high diagnostic value as it has higher scores in sensitivity，specificity，accuracy，positive likelihood ratio and negative likelihood ratio in the diagnosis of breast diseases.