Objective：To investigate the influence of bowel preparation on uterine diffusion weighted imaging magnetic susceptibility artifact. Methods：Totally 60 healthy women（ranged between 23 to 45 years old） were randomly divided into two groups：non bowel preparation group and bowel preparation group，30 subjects in each group. Then every subject was scanned for T2WI（axial and sagit－tal plane） and DWI. Fnally every image was evaluated by two doctors in a double-blind way. Results：In non bowel preparation group，average occurrence rate of air predominance was 65.00% and occurrence rate of fluid predominance was 35.00%. In bowel prepara－tion group，mean occurrence rate of air predominance was 23.33% and occurrence rate of fluid predominance was 76.67%. There were significantly statistical differences in two doctors’ evaluation between two groups（P1=0.002，P2=0.004）. The average positive rate for uterine DWI susceptibility artifact was 82.91% when fluid predominating in the intestinal tract and the average positive rate for uterine DWI susceptibility artifact was 13.46% when air predominating in the intestinal tract. There were significantly statistical differences in two doctors’ evaluation of magnetic susceptibility artifact between two groups（P1=0.000，P2=0.000）. Conclusion：Bowel preparation can decrease susceptibility artifact before uterine DWI examination，and then improve the quality of uterine DWI，which is beneficial for the uterine histological study as well as the diagnosis，monitoring and assessment of uterine diseases.