Objective To explore the effect of the problem-based learning（PBL） teaching methodology in the teaching of intern nursing students in the disinfection supply center. Methods A total of 122 intern nursing students in the disinfection supply center of a hospital in Zhengzhou from January 2018 to December 2020 were included in the study

and they were assigned to a control group（55 students

January 2018-July 2019） and an experimental group（67 participants

July 2019-January 2021）

according to the timeline. Nursing students in the control group were taught by the teacher’s explanation and operation demonstration

while the nursing students in the experimental group were taught by the PBL. The nursing students in the two groups were compared in professional evaluation results

comprehensive assessment and teaching satisfaction.Results The theoretical and skill assessment scores of nursing students in the experimental group were higher than those in the control group

and the differences were statistically significant（P<0.05）. The scores of nursing students in the experimental group were higher than those in the control group in terms of learning attitude

analyzing and solving problems

communication and verbal ability

and learning effect

and the differences were statistically significant（P<0.05）. The satisfaction of nursing students in the experimental group in terms of learning perception

academic perception

teaching environment perception and social perception were all higher than those in the control group

and the differences were statistically significant（P<0.05）. Conclusion The use of PBL teaching methodology in the teaching of nursing students in the disinfection supply center is conducive to promoting the nursing students’ mastery of theoretical knowledge and operational skills

improving their comprehensive quality

and enhancing the teaching quality.