Optogenetics is one of the biggest breakthroughs in neurobiology in recent decades

which is a revolutionary approach to precise intervention of specific cell functions through the combination of optical and genetic engineering techniques. However

this technique involves the interdisciplinary theoretical knowledge and methods

and it requires expensive equipments and long experimental period. Thus

it is not convenient to carry out widely in experiment teaching for undergraduates except the virtual simulation teaching. In present virtual simulation experiment

we aim to learn the basic principle

operation and application of optogenetics and achieve the desired teaching effects by simulating the process of the virus package

simulating the process of the animal selection

surgery and virus stereotactic microinjection

simulating the expression process of photosensitive channel

the extracellular verification process of photosensitive channel function in vivo

and simulating the observation process that optogenetic inhibition of glutamate neurons in the amygdala affect animal fear behavior. In this paper

the content design of the script making of the virtual experiment has been discussed in the above ways.