Effect of Cisplatin , Mitoxantrone and  Cytosine Arabinoside  on  rabbit lens epithelial cells

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Effect of Cisplatin , Mitoxantrone and Cytosine Arabinoside on rabbit lens epithelial cells

更新时间：2024-01-17

- Effect of Cisplatin , Mitoxantrone and Cytosine Arabinoside on rabbit lens epithelial cells
- Journal of Chongqing Medical University Issue 1, Pages: 25-28(2001)
- 作者机构：
  
  1. 重庆医科大学临床学院眼科,重庆,400016
  2. 重庆医科大学基础医学院病理生理学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R776.1
- Published：2001，
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[1]彭惠,李平华,罗莉霞,骆云鹏,汤为学.顺铂、米托蒽醌及阿糖胞苷对兔晶体上皮细胞的作用[J].重庆医科大学学报,2001(01):25-28.

Effect of Cisplatin , Mitoxantrone and Cytosine Arabinoside on rabbit lens epithelial cells. [J]. Journal of Chongqing Medical University (1):25-28(2001)
[1]彭惠,李平华,罗莉霞,骆云鹏,汤为学.顺铂、米托蒽醌及阿糖胞苷对兔晶体上皮细胞的作用[J].重庆医科大学学报,2001(01):25-28. DOI：

Effect of Cisplatin , Mitoxantrone and Cytosine Arabinoside on rabbit lens epithelial cells. [J]. Journal of Chongqing Medical University (1):25-28(2001) DOI：

摘要

目的：研究顺铂（Cisplatin ，PDD），米托蒽醌（Mitoxantrone， Mit），阿糖胞苷（Cytosine Arabinoside， Ara-C）对兔晶体上皮细胞（rabbit lens epithelial cells

RLECs）増殖的抑制作用。方法：分离兔晶体囊膜行组织细胞培养，传代的2～3代RLECs在96孔板培养24h，然后用不同浓度的PDD、Mit及Ara-c作用细胞24h及72h，用MTT（methyl thiazolyl tetrazolium）比色法进行测定。电镜观察细胞超微结构，流式细胞仪观察细胞周期的变化。结果：PDD及Mit作用于传代培养RLECs 24h IC50分别为38.92μg/ml 、8.92μg/ml ；72h 的 IC50 分别为6.71μg/ml、2.04μg/ml。结论：PDD及Mit均为剂量依赖型和时间依赖型药物，均能有效抑制兔晶体上皮细胞的生长，在此试验条件下Ara-C对兔晶体上皮细胞增殖无明显抑制作用。

Abstract

Objective: To study the ability of Cisplatin

Mitoxantrone and Cytosine Arabinoside on inhibiting the proliferation of rabbit lens epithelial cells(RLECs). Methods: Lens epithelium was isolated and cultured

and 2~3 passage RLECs were placed in 96-well flat bottomed tissue culture plates and incubated for 24 hours

then exposed to different concentrations of Cisplatin

Mitoxantrone and Cytosine Arabinoside for 24 and 72 hours and the effect of RLECs were measured with methyl thiazolyl tetrazolium (MTT) assay. The apoptosis of cells were observed with transmission electron microscope (TEM) .The changes of cell period were observed with flow cytometer (FCM). Results: The IC50 of cisplatin and mitoxantrone exposed to RLECs for 24 hours were 38.92μg/ml 、8.92μg/ml and those of 72 hour were 6.71μg/ml、2.04μg/ml respectively. Conclusion: Cisplatin and Mitoxantrone were dose- dependent and time-dependent inhibitors. Both of them inhibited the proliferation of RLECs effectively

Cytosine Arabinoside

however

had no obvious effect on RLECs in this experimental condition.

关键词

兔晶体上皮细胞抑制顺铂米托蒽醌阿糖胞苷

Keywords

Rabbit lens epithelial cellsinhibitioncisplatinMitoxantroneCytosine Arabinoside

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