Objective: To find some active materials that can be easily and cheaply attained and have similar function of hematopoietic growth factor(HGF). Methods: The technique of cell culture ex vivo and the assay of the biological activity of HGF were used. The supernatants of culture conditional media in fetal muscle tissue

thymocyte

spleen cell and human bone marrow stromal cell were collected. The effects of the different conditional media on the proliferation and differentiation of hematopoietic progenitor cell including CFU-GM

CFU-Mix and BFU-E were observed. Results: The culture conditional media of fetal muscle can markedly improve the proliferation and differentiation of CFU-GM and CFU-Mix; and the culture conditional media of thymocyte and spleen cell can enhance the growth of BFU-E. Conclusion: There may be some active materials similar GM-CSF in the supernatants of the bone marrow stromal cell and fetal muscle conditional media. The supernatants of thymocyte and spleen cell conditional media may function as the alike activity of IL-3. Such culture conditional media may act as the substitute for HGF in hematopoietic progenitor cell culture and in the clinic diagnostic assays.