Microvascular endothelial cell culture of Wistar rat cerebral cortex

WU Wenbin; et al

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Microvascular endothelial cell culture of Wistar rat cerebral cortex

更新时间：2024-01-17

- Microvascular endothelial cell culture of Wistar rat cerebral cortex
- Journal of Chongqing Medical University Issue 2, Pages: 151-152(2002)
- 作者机构：
  
  1. 重庆医科大学第二临床学院神经内科
  2. 重庆医科大学基础医学院病理生理教研室,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q813.1
- Published：2002，
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[1]吴文斌,胡常林,汤为学.大鼠脑皮质微血管内皮细胞的培养[J].重庆医科大学学报,2002(02):151-152+155.

WU Wenbin, et al. Microvascular endothelial cell culture of Wistar rat cerebral cortex. [J]. Journal of Chongqing Medical University (2):151-152(2002)
[1]吴文斌,胡常林,汤为学.大鼠脑皮质微血管内皮细胞的培养[J].重庆医科大学学报,2002(02):151-152+155. DOI：

WU Wenbin, et al. Microvascular endothelial cell culture of Wistar rat cerebral cortex. [J]. Journal of Chongqing Medical University (2):151-152(2002) DOI：

摘要

目的:培养脑皮质微血管内皮细胞。方法:取～乳鼠脑皮质经不同孔径的筛网过滤后

用胶原酶振荡消15d Wistar

化获得的微血管内皮细胞进行培养

用Ⅷ因子相关抗原免疫组化鉴定。结果:培养的细胞呈单层贴壁生长

～呈典型的铺79d路卵石样征象。结论:建立了一种简便易行的培养脑皮质微血管内皮细胞的方法。

Abstract

Objective: To culture microvascular endothelial cells from Wistar rat cerebral cortex. Methods: Cerebral cortex from Wistar rats of 1~5days filtered by various diameter meshes. The pellets containing the microvessels were washed and collected by centrifugation.The microvessels obtained from above procedures were Ⅶdigested by collagenase .The isolated endothelial cells were cultured in M199 +15% fetal calf serum at a density ×of 3104/ml and observed morphologically. Results:The cultured microvascular endothelial cells were stained Ⅷpositively for factor relative antigen. On the 7~9th day

the cells displayed monolayer growth and looked like "cobblestones"

a typical appearance of cultured endothelial cells under inverted microscope. Conclusion: It is a simple and easy method to culture endothelial cells from brain microvessels.

关键词

细胞培养微血管内皮细胞Wistar大鼠

Keywords

cell culturemicrovascular endothelial cellWistar rat

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