Investigations on cytoskeleton of K562 cells induced by matrine

ZHANG Yan; et al

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Investigations on cytoskeleton of K562 cells induced by matrine

更新时间：2024-01-17

- Investigations on cytoskeleton of K562 cells induced by matrine
- Journal of Chongqing Medical University Issue 4, Pages: 386-387(2002)
- 作者机构：
  
  1. 重庆医科大学检验系临床生化教研室
  2. 重庆医科大学检验系临床生化教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2002，
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[1]张彦,刘小珊,许相儒,刘北忠,何於娟,马凌娣,蒋纪恺.苦参碱对K562细胞骨架的作用观察[J].重庆医科大学学报,2002(04):386-387+390.

ZHANG Yan, et al. Investigations on cytoskeleton of K562 cells induced by matrine. [J]. Journal of Chongqing Medical University (4):386-387(2002)
[1]张彦,刘小珊,许相儒,刘北忠,何於娟,马凌娣,蒋纪恺.苦参碱对K562细胞骨架的作用观察[J].重庆医科大学学报,2002(04):386-387+390. DOI：

ZHANG Yan, et al. Investigations on cytoskeleton of K562 cells induced by matrine. [J]. Journal of Chongqing Medical University (4):386-387(2002) DOI：

摘要

目的：从细胞骨架方面进一步探索苦参碱对人白血病K562细胞株的作用机制。方法：采用微管吮吸技术和基因芯片技术分析苦参碱处理前后K562细胞粘弹系数和细胞骨架蛋白基因表达的改变。结果：0.1mg/ml苦参碱作用K562细胞24h后粘弹系数下降，细胞骨架类基因prefoldin 与ezrin 的mRNA表达增强。结论：苦参碱能够导致K562细胞的细胞骨架发生变化。

Abstract

Objective: To study the effect of matrine on cytoskeleton of K562 cells. Methods: Micropipette aspiration technique was adopted to investigate the viscoelasticity of K562 cells

while the different expression of cytoskeletal protein gene was analyzed by DNA microarray. Results: In matrine-treated K562 cells

the viscoelastic properties K1，K2 and μwere decreased significantly

and the gene expression of prefoldin and ezrin was much stronger than that of control cells. Conclusion: The cytoskeleton changed in matrine-treated K562 cells.

关键词

苦参碱基因芯片细胞骨架K562细胞

Keywords

MatrineDNA microarraycytoskeletonK562 cells

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Related Institution

重庆医科大学临床学院血液科

Department of Clinical Biochemistry, Chongqing Medical University

重庆医科大学基础医学院临床生化教研室

重庆市永川市人民医院

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