Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR

XIA Yun; et al

您当前的位置：

首页 >

文章列表页 >

Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR

更新时间：2024-01-17

- Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR
- Journal of Chongqing Medical University Issue 1, Pages: 78-80(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院检验科
  2. 重庆合川市人民医院,重庆,400016
  3. ,重庆,401520
- 作者简介：
- 基金信息：
- DOI：
  CLC： R378
- Published：2006，
扫描看全文
[1]夏云,李明爽.应用重复序列PCR技术对嗜麦芽窄食单胞菌进行基因分型[J].重庆医科大学学报,2006(01):78-80+84.

XIA Yun, et al. Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR. [J]. Journal of Chongqing Medical University (1):78-80(2006)
[1]夏云,李明爽.应用重复序列PCR技术对嗜麦芽窄食单胞菌进行基因分型[J].重庆医科大学学报,2006(01):78-80+84. DOI：

XIA Yun, et al. Genotyping of Stenotrophomonas maltophilia by using repeat sequence PCR. [J]. Journal of Chongqing Medical University (1):78-80(2006) DOI：

摘要

目的:建立一种快速、简便、准确的基因分型方法

对嗜麦芽窄食单胞菌的感染进行流行病学调查

确定基因型与耐药模式之间的相关性。方法:选用肠杆菌科基因重复序列（ERIC）为引物进行PCR扩增

对14株嗜麦芽窄食单胞菌进行基因分型研究

并与其抗生素耐药结果进行比较。结果:14株嗜麦芽窄食单胞菌呈现9种耐药模式

其中13株菌表现为5种基因型。表明嗜麦芽窄食单胞菌的耐药模式和基因型之间无明确的相关性。结论:ERIC-PCR应用简便易行

重复性好

适合于临床分离的病原菌的分型及医院感染的分子流行病学研究。

Abstract

Objective: To establish a rapid

simple and accurate genotyping method for the epidermical investigation of nosocomial infection caused by Stenotrophomonas maltophilia

and to explore the relationship between the genotyping and the drug-resistant profiles.Methods: Fourteen strains of Stenotrophomonas maltophilia isolated from clinic were genotyped by the techenic of Rep-PCR with the primer of enterobacterial repetitive intergenic consensus(ERIC)

and the results of Rep-PCR were analyzed and compared with the patterns of antibiotic susceptibility.Results: All 14 strains involved in this study exhibited nine antibiotic susceptibility patterns.Of which 13 strains exhibited 5 different genotypes.There were no defined relationship between the results of the genotyping and the drug-resistant profiles.Conclusion: The Rep-PCR techenic used in this study is simple and repreducible for genotyping of Stenotrophomonas maltophilia.It is valuable in the molecular epidemiological investigation of nosocomial infection.

关键词

嗜麦芽窄食单胞菌聚合酶链反应基因分型

Keywords

Stenotrophomonas maltophiliaPolymerase chain reactionGenotyping.

references

Views

143

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Glycogen assay for diagnosis of female genital Chlamydia trachomatis infection

Overcome false - positive in detection of tumor microme- tastasis by reverse transcriptase - polymerase chain reaction

Detection of Epstein- Barr virus withpolymerase chain reaction in diagnosis fornasopharyngeal carcinoma

Preparing heavy and light chain variable region genes for anti- telomerase protein hTERT by the polymerase chain reaction

Clinicopathologic value of detection of esophageal squamous carcinoma micrometastases in regional lymph nodes by means of reverse transcriptase-polymerase chain reaction

Related Author

朱道银

杨春

黄天禄

王梅

张玉洪

李良彬

葛明建

李素芳

Related Institution

重庆医科大学基础医学院微生物学教研室重庆医科大学基础医学院微生物学教研室讲师

重庆医科大学基础医学院微生物学教研室讲师

重庆医科大学基础医学院微生物学教研室

Department of Otolaryngology, College of Clinical Medicine

北京医科大学病理学教研室

⁰