Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX<sub>2</sub> carcinoma rabbit model

CHEN Xiang; et al

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Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX₂ carcinoma rabbit model

更新时间：2024-01-17

- Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX₂ carcinoma rabbit model
- Journal of Chongqing Medical University Issue 1, Pages: 74-77(2006)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肝胆外科
  2. 重庆医科大学附属第一医院肝胆外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2006，
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[1]陈翔,王洪林,李坚,涂刚,张盟辉.射频联合化疗药物治疗兔肝VX_2肿瘤模型的实验研究[J].重庆医科大学学报,2006(01):74-77.

CHEN Xiang, et al. Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX₂ carcinoma rabbit model. [J]. Journal of Chongqing Medical University (1):74-77(2006)
[1]陈翔,王洪林,李坚,涂刚,张盟辉.射频联合化疗药物治疗兔肝VX_2肿瘤模型的实验研究[J].重庆医科大学学报,2006(01):74-77. DOI：

CHEN Xiang, et al. Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX₂ carcinoma rabbit model. [J]. Journal of Chongqing Medical University (1):74-77(2006) DOI：

摘要

目的:观察射频联合化疗药物治疗兔肝VX2肿瘤的疗效

并探讨其作用机制。方法:建立动物模型

随机分为四组

观察血清学ALT﹑肿瘤直径﹑H E染色等变化情况

运用免疫组化和TUNEL法检测细胞增殖和凋亡情况。结果:⑴ALT:联合治疗组后第1天明显升高

第3天后开始下降

1w后已降至治疗前水平下;（2）肿瘤直径:联合治疗组较其他组有显著差异（P<0.05）;（3）病理形态学观察:联合治疗组H E染色可见正常肝组织﹑炎症充血带﹑无定形的坏死组织形成的条带;（4）细胞凋亡和增殖:联合治疗组的凋亡指数较其他组高（P<0.05）

而增殖指数较其他组低（P<0.05）

两者之间呈负相关（r=-0.745

P<0.01）。结论:射频联合化疗药物治疗肝VX2肿瘤疗效比较明显

对肝功能损伤较小;其治疗机制可能为诱导细胞凋亡同时抑制细胞增殖。

Abstract

Objective: To observe the therapeutic effects of RFA combined with chemotherapy drug on hepatic VX: carcinoma in rabbits and to explore its mechanism.Methods: 40 rabbit models with VX: carcinoma were used for this study.they were divided into four groups at random.We watched the changes in serolog ALT

diameter of tumor and pathomorphology.And we also utilized terminal deoxynucleotidyl ransferase-mediated dUTP nick-end-labeling and immunohistochemistry to observe the apoptosis and proliferation.Results: ⑴The blood ALT significantly increased at the first day after treatment

then descended at the third day

and at the seventh day it descended to the level of pretreatment stage in group RFA combined with epirubicin.(2) The diameter of tumor showed significant difference between combined treatment and other groups.(3) Two weeks after treatment

the complete necrotic area slightly extended.it was clearly seen that the zone consisted of normal liver constitution

inflammation hyperemia zone and amorphous necrotic constitution by HE staining in combined treatment group.(4) The apoptosis index of the combined treatment group was bigger than that in other groups by TUNEL technology

but the PCNA index in the combined treatment group was lower than other groups.They all had significant difference.There was negative correlation between apoptosis index and PCNA index in the combined treatment group(r=-0.745

P<0.01).Conclusion: The therapeutic effects of RFA combined with chemotherapy drug for treatment of VX2 carcinoma in rabbit model is reliable with little injury to liver function.The mechanism may be that the combined treatment induces the apoptosis and inhibits the cell proliferation.

关键词

射频消融化疗药物增殖细胞核抗原细胞凋亡

Keywords

Radio-frequency ablationChemotherapy drugProliterating cell nuclear antigenapoptosis

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Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX2 carcinoma rabbit model

DOI：

摘要

Abstract

关键词

Keywords

references

Experimental study on radio frequency ablation（RFA） combined with chemotherapy drug for treatment of Hepatic VX₂ carcinoma rabbit model