Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells

HU Huaidong; et al

您当前的位置：

首页 >

文章列表页 >

Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells

更新时间：2024-01-17

- Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells
- Journal of Chongqing Medical University Issue 6, Pages: 801-803(2006)
- 作者机构：
  
  1. 重庆医科大学附属第二医院内分泌科
  2. 重庆医科大学附属第二医院神经内科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.2
- Published：2006，
扫描看全文
[1]胡怀东,陈莉芬,胡长林.高糖对脑微血管内皮细胞活力和内皮素-1的影响[J].重庆医科大学学报,2006(06):801-803+807.

HU Huaidong, et al. Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells. [J]. Journal of Chongqing Medical University (6):801-803(2006)
[1]胡怀东,陈莉芬,胡长林.高糖对脑微血管内皮细胞活力和内皮素-1的影响[J].重庆医科大学学报,2006(06):801-803+807. DOI：

HU Huaidong, et al. Effects of high glucose concentration on endothelin-1 and activity of cultured brain microvascular endothelial cells. [J]. Journal of Chongqing Medical University (6):801-803(2006) DOI：

摘要

目的:探讨体外培养条件下高糖状态对大鼠脑皮质微血管内皮细胞（Brain microvascular endothelial cells

BMEC）的影响。方法:建立大鼠脑皮质微血管内皮细胞体外培养模型

用透射电镜观察正常浓度（5.05mm ol/L）和高浓度（15mmol/L、30mmol/L）葡萄糖培养基中培养24h后内皮细胞的超微结构变化

用M TT法评价内皮细胞活力

采用放射免疫方法测定内皮素-1（Endothelin-1

ET-1）含量

用原位杂交法测定ET-1mRNA表达。结果:高浓度葡萄糖可使内皮细胞超微结构损伤

细胞活力降低

ET-1分泌显著上调

并呈剂量依赖性损伤细胞

但ET-1 mRNA表达无明显变化。结论:高糖对脑皮质微血管内皮细胞有显著的损伤作用

ET-1升高在糖尿病微血管病变早期可能起主要作用。

Abstract

Objective:To investigate the effects of high glucose on rat brain microvascular endothelial cells(BMEC).Methods: Brain microvascular endothelial cells(BMEC)of Wistar rat cerebral cortex were cultured in 5.05mmol/L glucose

15 and 30mmol/L glucose media respectively for 24h

and then BMEC activity or injury was assayed by observing cell ultramicrostructures morphology and adopting methyl thiazolyl tetrazolium(MTT)assay.The ET-1 concentrations were evaluated by radioimmunoassay.Situ hybridization used to detect the ET-1 mRNA expression.Results:The BMEC activity and viability assessed by MTT were decreased when glucose concentrations rose.The ultrastructure of BMEC was damaged and the content of ET-1 in BMEC media was increased.Furthermore

ET-1 mRNA expression was not induced by high concentration of glucose.Conclusions:High concentration of glucose has a significant effect of BMEC injury.The data suggest that ET-1 may play important role in the early state of diabetic microvascular complications.

关键词

脑微血管内皮细胞高糖内皮素-1

Keywords

Brain microvascular endothelial cellshigh glucoseendothelin-1

references

Views

175

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Effects of HIF1α-KIM1 signaling pathway on renal interstitial fibrosis in diabetic nephropathy

Research on the correlation between the plasma endothelin-1 level and chronic lower extremities ischemia

Changes of plasm endothelin-1 in rabbits with alcoholism

Protective effect of sodium ferulate on rat brain microvascular endothelial cells injured by hypoxia

Effect of PFT-α on ubiquitin and apoptosis of podocyte injured by high glucose

Related Author

Dang Yong

Li Yaqian

Yu Fang

时德

et al

REN Wei

XIE Juan

et al

Related Institution

The First Clinical Medical College of Nanchang University

Department of Nephrology,Anyang People’s Hospital Henananyang

重庆医科大学临床学院普外科

重庆医科大学检验系

⁰