Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide

WU Hong; et al

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Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide

更新时间：2024-01-17

- Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide
- Journal of Chongqing Medical University Issue 6, Pages: 804-807(2006)
- 作者机构：
  
  1. 重庆医科大学基础医学院组胚教研室
  2. 重庆医科大学基础医学院组胚教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2006，
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[1]吴宏,赵俊,李芳菲,王亚平.人参多糖动员的造血干/祖细胞移植重建造血衰竭小鼠造血功能的研究[J].重庆医科大学学报,2006(06):804-807.

WU Hong, et al. Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide. [J]. Journal of Chongqing Medical University (6):804-807(2006)
[1]吴宏,赵俊,李芳菲,王亚平.人参多糖动员的造血干/祖细胞移植重建造血衰竭小鼠造血功能的研究[J].重庆医科大学学报,2006(06):804-807. DOI：

WU Hong, et al. Study on hematopoietic reconstruction for bone marrow depression mice with the transplantation of mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide. [J]. Journal of Chongqing Medical University (6):804-807(2006) DOI：

摘要

目的:探讨经人参多糖动员的外周血造血干/祖细胞移植对造血衰竭小鼠造血重建的作用。方法:建立小鼠造血衰竭模型

移植经人参多糖动员的外周血单个核细胞

检测受体小鼠生存时间

外周血WBC

脾湿重及脾集落数

进行染色体嵌合实验。结果:经人参多糖动员的雄性小鼠外周血MNC1×105个输注给致死剂量照射的雌性小鼠

可明显延长小鼠存活时间;提高受体小鼠外周血WBC数;亦能增加脾湿重

提高脾集落（CFU-S）数;经Y染色体检测（PCR法）受体小鼠外周血单个核细胞可检出581bpY染色体序列。结论:经人参多糖动员小鼠的外周血中存在数量较多的造血干/祖细胞

回输给造血衰竭小鼠能重建小鼠造血功能。

Abstract

Objective:To study the hematopoietic reconstruction for bone marrow depression mice with mobilized peripheral blood stem/proginiter cells by ginseng polysaccharide.Methods: Establish hematopoiesis failure model of mouse and transplant murine peripheral blood mononuclear cells mobilized by GPS.To observe the survival time

WBC

the weight of spleen

the number of CFU-S of recipient mice and chromosome chimerism.Results: After PBMNCs(1×105)mobilized by GPS from mice(male) were injected to the lethality irradiated mice

it can reduce the time of WBC recovery obviously;prolong the time of murine survival

increase the number of WBC of the recipient mice;it can also increase the number of CFU-S

the weight of spleen;Evaluating the Y chromosome by PCR

the result showed that 581bp Y chromosome sequence was examined in peripheral blood of the recipient mice.Conclusion: There are a lot of hematopoietic stem/ proginiter cells in peripheral blood of the mice mobilized by GPS

so it can obviously reconstruct the hematopoiesis of the bone marrow depression mice by transplantation.

关键词

人参多糖动员造血干/祖细胞移植造血重建

Keywords

Ginseng polysaccharidemobilizationHematopoietic stem/ progenitor celltransplantationHematopoietic reconstruction

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