Objective:To explore the proliferation regularity of different gestational age human embryonic neural stem cells after them were isolated

cultivated and purified successfully.Methods:3~18 weeks human embryo were obtained under asepsis condition

and divided into 3 groups according to gestation age: A group(3~6w)

B group(7~11w)

C group(12~18w).The each group of NSCs were isolated and purified after primary culture

and identified by single-cell clone culture and immunocytochemistry.(ICC)To study NSCs growth feature by counting numbers and diameters of neurospheres. Results:The cells could survive and proliferate in vitro which were isolated from 3~6 weeks human embryo pallium and neurocanal or 7~18 weeks human fetus pallium. Identification of the cells showed that they had the typical feature of NSC biology in many aspects

such as growth pattern

morphosis

function feature and surface marker. The numbers and diameters of neurospheres from 3~6w gestation age group are much higher than that from 7~18w gestation age group(P<0.05). Conclusion:Succeeded in isolating cells this can survive in vitro for long time from 3~18 weeks human embryo pallium and neurocanal. Culture in vitro and identification showed the cells were NSCs.There were significant differences in proliferation characteristic of each group

The proliferation characteristics of little gestation age groups better than big gestation age groups.