Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms

WANG Delin; et al

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Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms

更新时间：2024-01-17

- Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms
- Journal of Chongqing Medical University Issue 4, Pages: 362-365(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学基础医学院病理教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.11
- Published：2007，
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[1]王德林,米粲,陈在贤,吴小候,苟欣.As_2O_3对肾癌细胞786-0细胞周期的影响及其机制的研究[J].重庆医科大学学报,2007(04):362-365.

WANG Delin, et al. Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms. [J]. Journal of Chongqing Medical University (4):362-365(2007)
[1]王德林,米粲,陈在贤,吴小候,苟欣.As_2O_3对肾癌细胞786-0细胞周期的影响及其机制的研究[J].重庆医科大学学报,2007(04):362-365. DOI：

WANG Delin, et al. Influences of arsenic trioxide on the cell cycle of human renal cell carcinoma 786-0 cells in vitro and its mechanisms. [J]. Journal of Chongqing Medical University (4):362-365(2007) DOI：

摘要

目的:探讨三氧化二砷（As2O3）对人肾癌细胞系786-0的细胞周期、周期素（Cyclin）和周期素依赖性激酶抑制剂（DKI）的影响

探讨As2O3抗肾癌作用的机制。方法:采用细胞培养、流式细胞技术观察As2O3作用786-0细胞后细胞周期的改变

以免疫细胞化学和RT-PCR技术等检测As2O3致细胞Cyclin蛋白和CDKI基因的改变。结果:以2μmol/L或5μmol/LAs2O3作用786-0细胞24h、48h、72h

使其细胞周期停滞于G2/M和G0/G1期

并出现细胞凋亡

周期素蛋白CyclinA、B1、D1、E表达均不同程度降低（P<0.05）

而其CDKI基因p16、p21WAF/CIPI、p27kip1表达升高（P<0.05）。结论:As2O3通过降低CyclinA、B1、D1、E蛋白的表达和增强p16、P21WAF/CIPI、p27kip1活性

将786-0细胞阻滞于G2/M和G0/G1期

达到其抑制增殖和诱导细胞凋亡的作用。

Abstract

Objective:To explore the influence of arsenic trioxide on cell cycle

cyclin and cyclin-dependent kinase inhibitor(CDKI) of human renal cell carcinoma 786-0 cells.Methods:The change of cell cycle was measured by flow cytometric assay(FCM)

and its mechanism was analyzed by immunocytochemistry and reverse transcription polymerase chain reaction. Results: Treatment with As2O3 of 2μmol/L or 5μmol/L for 24h

48h and 72h

notably induced a G2/M and G0/G1 cell cycle arrest in 786-0 cells

decreased expression of cyclin A

E(P<0.05) and increased expressions of CDKI genes p16

P21WAF1/CIPI and p27kip1(P<0.01).Conclusions:As2O3 can inhibit the growth in 786-0 cells effectively by down-regulating expression of cyclinA

E and up-regulating expressions of p16

P21WAF1/CIPI and p27kip1 genes.

关键词

As2O3肾癌786-0细胞细胞周期凋亡

Keywords

Arsenic trioxiderenal cell carcinoma786-0 cellscell cycleapoptosis

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