Objective:To observe the change of myocardial cell and matrix in the rats with type 2 diabetes and to approach the construction method of diabetic cardiomyopathy models in wistar rats with type 2 diabetes mellitus.Method:Sixteen wistar rats of 6 months were randomly divided into normal group(N)and diabetic group(D)

with eight rats in each group.Euglycemic insulin clamp technique(EICT)was used to determine insulin resistance(IR).Heart weight(HW)and body weight(BW)of each rat were measured to ratio HW and BW

The apoptosis of myocardial cell was evaluated by TUNEL.

and the ultramicrostructure of.myocardial cell and construction of heart were observed.And immunohistochemistry (SABC method) was used to measure the level of Ⅰand Ⅲ types of collagen protein.Results:compared with group(N)

GIR of group(D) was remarkably lower(P<0.01)

the ratio of HW and BW was higher

and the apoptosis of myocardial cell increased(P<0.01).For Group D

optical microscopy observed the disorder of rat myocardial cells

and the assembling and fibrosis change of interstitial matrix.And electromicroscopy observed slight swelling of myocardial cell nucleus

the scattered distribution of chromatin under nuclear membrane

swelling of mitocliondrion and dissolution of myofilament

And levels of Ⅰand Ⅲ types of collagen protein in group D increased remarkably(P<0.01).Conclusion:Wistar rats with type 2 diabetes mellitus presented pecthological change of DC 8 weeks after modeling

which can be used to study diabetic cardiomyopathy.