Expression of Ngn3 in the donor islets with different purity

XUE Dong; et al

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Expression of Ngn3 in the donor islets with different purity

更新时间：2024-01-17

- Expression of Ngn3 in the donor islets with different purity
- Journal of Chongqing Medical University Issue 1, Pages: 12-14(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院肝胆暨肝移植外科
  2. 重庆医科大学附属第一医院肝胆暨肝移植外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R657.5
- Published：2007，
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[1]薛栋,杜成友,杨闯,尹昌生.神经元素3在不同纯度供体胰岛细胞中的表达[J].重庆医科大学学报,2007(01):12-14+21.

XUE Dong, et al. Expression of Ngn3 in the donor islets with different purity. [J]. Journal of Chongqing Medical University (1):12-14(2007)
[1]薛栋,杜成友,杨闯,尹昌生.神经元素3在不同纯度供体胰岛细胞中的表达[J].重庆医科大学学报,2007(01):12-14+21. DOI：

XUE Dong, et al. Expression of Ngn3 in the donor islets with different purity. [J]. Journal of Chongqing Medical University (1):12-14(2007) DOI：

摘要

目的:研究神经元素3（Ngn3）在不同纯度胰岛细胞中的表达情况

探讨不同纯度胰岛细胞中所携带的干细胞。方法:30只成年雄性SD大鼠随机分为3组

采用胰管内注入胶原酶消化法分离成年大鼠胰腺

Ⅰ组（n=10）:采用2次消化、不纯化的方法获得胰岛;Ⅱ组（n=10）:胰岛沉淀物加入25%Ficoll-400纯化

Ⅲ组（n=10）:胰岛沉淀物依次加入25%、11%的Ficoll-400纯化。应用免疫组化、逆转录-多聚酶链反应（RT-PCR）方法进行定位、半定量分析

检测Ngn3在不同纯度胰岛细胞中的表达。结果:Ⅰ组获得胰岛纯度约为43.6%±6.29%

Ⅱ组获得胰岛纯度约为65.3%±4.40%

Ⅲ组获得胰岛纯度约为77.6%±6.36%

各组比较均具有显著性差异（P<0.05）。Ngn3在不同纯度胰岛细胞中均有表达

Ⅰ组中Ngn3的蛋白和和核酸水平表达明显高于Ⅱ组、Ⅲ组

Ⅲ组中表达最弱

各组比较均具有显著性差异（P<0.05）。结论:Ngn3在不同纯度胰岛细胞中均有表达

在低纯度胰岛细胞中表达最明显

提示低纯度胰岛细胞中可能携带较多的干细胞。

Abstract

Objective:To study the expression of Ngn3 in rats donor islets with different purity

and explore the stem cells in them.Methods:Thirty male adult SD rats were randomly divided into 3 groups.Islets were isolated by digestion method of injecting collagenase into pancreatic duct.Group I was harvested by two digestions without purification;For group Ⅱ(n=10)

islets sediments were purified by adding 25% ficoll-400 and for

Group Ⅲ(n=10)

by adding (25% and 11%)ficoll-400 in order.Finally we applied immunohistochemisiry and RT-PCR to the location and semi-quantitative analysis to test the expression of Ngn3 in the donor islets with different purity.Results:Islets purity of group I was 43.6%±6.29%

Group II 65.3%±4.40%

and group Ⅲ77.6%±6.36%.The difference of Islet purity was significant in three groups (P<0.05).Compared with group Ⅱ and group Ⅲ

the expressions of protein and mRNA of Ngn3 were both highest in group Ⅰ

lowest in group Ⅲ(P<0.05).Conclusion:Ngn3 was expressed in all islets

with the low purity expressing the most prominent

which suggests that islets with low purity may bear more stem cells.

关键词

神经元素3供体胰岛逆转录-多聚酶链反应免疫组化

Keywords

Ngn3Donor isletRT-PCRimmunohistochemistry

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