Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens

LIU Chonghai; et al

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Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens

更新时间：2024-01-17

- Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens
- Journal of Chongqing Medical University Issue 1, Pages: 40-43(2007)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院免疫实验室
  2. 重庆医科大学附属儿童医院免疫实验室,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R-332
- Published：2007，
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[1]刘崇海,杨锡强,王莉佳,何云.屋尘螨诱导肠道菌群失调小鼠变应性气道反应模型的建立[J].重庆医科大学学报,2007(01):40-43+58.

LIU Chonghai, et al. Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens. [J]. Journal of Chongqing Medical University (1):40-43(2007)
[1]刘崇海,杨锡强,王莉佳,何云.屋尘螨诱导肠道菌群失调小鼠变应性气道反应模型的建立[J].重庆医科大学学报,2007(01):40-43+58. DOI：

LIU Chonghai, et al. Establishment of allergic airway response model in mice with intestinal microflora imbalance induced by dermatophagoides pteronyssinus allergens. [J]. Journal of Chongqing Medical University (1):40-43(2007) DOI：

摘要

目的:在肠道菌群失调小鼠模型上采用屋尘螨变应原诱导气道变应性

建立一种新的变应性气道反应模型。方法:60只BALB/c小鼠分6组

2组用于肠道菌群检测;其余4组用于气道变应性检测。结果:抗生素组小鼠出现肠道菌群失调伴体重减少

粪便含水量增加。肠道菌群失调后进行屋尘螨变应原激发的小鼠BALF中细胞总数、淋巴细胞、中性粒细胞和嗜酸粒细胞增加

IL-4水平增高

肺部GATA-3转录因子mRNA表达增加。结论:用屋尘螨变应原在肠道菌群失调小鼠模型上气道激发

诱导小鼠产生Th2细胞优势的变应性气道反应。

Abstract

Objective:To establish the intestinal microflora imbalance mouse model to construct a novel allergic airway response model induced by dermatophagoides pteronyssinus allergens. Methods:Sixty BALB/c mice were divided into six groups. Mice in two groups were prepared for the analysis of the quantity of the intestinal bacterial flora and mice in other four groups for the analysis of allergic airway response. Results:Intestinal microflora of mice in antibiotic therapy group presented the weight loss and feces moisture increase. For the mice with intestinal microflora imbalance and induced by dermatophagoides pteronyssinus allergens

the number of total cells

lymphocytes

neutrophils and eosinophils in bronchoalveolar lavage fluid(BALF)significantly increased

the expression level of IL-4 increased

and the expression of GATA-3 mRNA in the lung tissue were upregulated. Conclusions:An allergic airway response model was established successfully by using dermatophagoides pteronyssinus allergens to induce mice with intestinal microflora disruption.

关键词

屋尘螨肠道菌群气道变应性动物模型

Keywords

Dermatophagoides pteronyssinusIntestinal microfloraAllergic airway responseanimal model

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