Expression of BUB1 gene in trophoblastic cells was depressed by microRNA

SHI Qiong; et al

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Expression of BUB1 gene in trophoblastic cells was depressed by microRNA

更新时间：2024-01-17

- Expression of BUB1 gene in trophoblastic cells was depressed by microRNA
- Journal of Chongqing Medical University Issue 1, Pages: 44-47(2007)
- 作者机构：
  
  1. 重庆医科大学临床检验诊断学实验室
  2. 重庆医科大学临床检验诊断学实验室基础医学院核医学教研室
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R346
- Published：2007，
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[1]施琼,翁亚光,徐远久,蔡燕,蒋洪彦.微小RNA对胚胎细胞BUB1基因的表达抑制[J].重庆医科大学学报,2007(01):44-47+51.

SHI Qiong, et al. Expression of BUB1 gene in trophoblastic cells was depressed by microRNA. [J]. Journal of Chongqing Medical University (1):44-47(2007)
[1]施琼,翁亚光,徐远久,蔡燕,蒋洪彦.微小RNA对胚胎细胞BUB1基因的表达抑制[J].重庆医科大学学报,2007(01):44-47+51. DOI：

SHI Qiong, et al. Expression of BUB1 gene in trophoblastic cells was depressed by microRNA. [J]. Journal of Chongqing Medical University (1):44-47(2007) DOI：

摘要

目的:探讨微小RNA对胚胎细胞BUB1基因表达的调控。方法:用定量real-timePCR比较microRNA转染前后的胚胎细胞内源性基因BUB1mRNA表达水平

同时用westernblot比较转染前后Bub1蛋白的表达水平。结果:对照组的BUB1基因mRNA拷贝数/GAPAHmRNA拷贝数为0.1960±0.0688

而转染microRNA重组质粒后

实验组的mRNA比值分别为0.1968±0.0689和0.1962±0.0670

经统计分析

两实验组细胞的内源性BUB1基因mRNA水平与对照组无显著性差异;而蛋白水平在转染后均有明显降低。结论:microRNA主要在翻译水平调节哺乳动物细胞内基因的表达

为研究基因表达的调控机制奠定了良好的基础。

Abstract

Objective:To discuss the depressed expression of BUB1 gene in trophoblastic cells by microRNA.Methods:Endogenous BUB1 mRNA level was compared by using quantitative real-time PCR before and after transfected with microRNA plasmid; and the Bub1 protein level was compared by western blot.Results:Ratio of BUB1 mRNA/GAPDH mRNA of control group was 0.1960±0.0688

and after transfected with microRNA recombinant plasmid was 0.1968±0.0689 and 0.1962±0.0670 respectively.Statistical study of samples showed they have no instinct different;otherwise the protein level was significantly decreased after transfected with microRNA plamid.Conclusion:This study established microRNA can control gene expression in mammalian cells at translation level

it is a good foundation to study on the control of gene expression.

关键词

微小RNABUB1基因定量RT-PCR

Keywords

microRNABUB1 geneQuantitative real-time PCR

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Related Institution

重庆医科大学临床检验诊断学实验室重庆医科大学临床检验诊断学实验室

重庆医科大学临床检验诊断学实验室

Department of nucleur,College of Basie medicine,Chongqing Medical University

Clinical Medical Research Center，the Second Clinical Medical College of Jinan UniversityShenzhen People’s Hospital

Department of Clinical Laboratory，The Seventh People’s Hospital of Chongqing

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