Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β<sub>7</sub> gene

WANG Yongming; et al

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Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β₇ gene

更新时间：2024-01-17

- Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β₇ gene
- Journal of Chongqing Medical University Issue 10, Pages: 1027-1031(2007)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院儿童保健科
  2. 重庆医科大学附属儿童医院儿童保健科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2007，
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[1]王永明,胡燕,黎海芪.小鼠整合素β_7基因克隆、序列分析及突变修复[J].重庆医科大学学报,2007(10):1027-1031.

WANG Yongming, et al. Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β₇ gene. [J]. Journal of Chongqing Medical University (10):1027-1031(2007)
[1]王永明,胡燕,黎海芪.小鼠整合素β_7基因克隆、序列分析及突变修复[J].重庆医科大学学报,2007(10):1027-1031. DOI：

WANG Yongming, et al. Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β₇ gene. [J]. Journal of Chongqing Medical University (10):1027-1031(2007) DOI：

摘要

目的:克隆小鼠整合素β7基因cDNA

同时对其序列进行分析

对造成氨基酸突变的碱基进行定点突变修复。方法:采用RT-PCR的方法

从小鼠小肠PP结获得β7基因cDNA

克隆到pMD19-T载体

选择阳性克隆进行酶切鉴定和序列测定

对造成氨基酸突变的碱基进行定点突变修复。结果:扩增得到的β7基因cDNA为2418bp

编码806个氨基酸

与GenBank中公布的序列比较

有10个碱基发生突变

其中造成氨基酸发生改变的有5个碱基

涉及3个氨基酸

对发生突变的5个碱基进行定点突变修复。结论:获得小鼠整合素β7基因的克隆

为进一步研究其生物学功能奠定了良好基础。

Abstract

Objective:To clone and analyze a full-length cDNA encoding mouse integrin β7

and repair the mutation of β7 cDNA that caused the change of amino acids. Methods:The cDNA of β7 gene was amplified by RT-PCR using the total RNA as extracted from mouse small intestine Peyer’s patch. The PCR product was inserted into pMD19-T vector and then transformed E.coli JM109. The positive recombinant clone was analyzed by restriction endonuclease and DNA sequencing. The mutation of β7 cDNA that caused the change of amino acids was repaired. Results:The cDNA of mouse β7 has an complete open reading frame with a length of 2418 bp

which encodes a product of 806 amino acid

and has 10 base pairs mutation of β7 gene and 5 base pairs mutation that caused the change of amino acids was repaired. Conclusion:The cDNA of mouse β7 was cloned successfully

which posed a basis for further researching on its biological function.

关键词

β7基因基因克隆RT-PCR突变修复

Keywords

β7 genecDNA cloneRT-PCRRepairing mutation

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重庆医科大学计划生育医学系

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Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β7 gene

DOI：

摘要

Abstract

关键词

Keywords

references

Molecular cloning,sequence analysising and repairing the mutation of mouse integrin β₇ gene