Study on AdCD-UPRT suicide gene mediated on lung cancer A<sub>549</sub> in vitro

XU Dan; et al

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Study on AdCD-UPRT suicide gene mediated on lung cancer A₅₄₉ in vitro

更新时间：2024-01-17

- Study on AdCD-UPRT suicide gene mediated on lung cancer A₅₄₉ in vitro
- Journal of Chongqing Medical University Issue 10, Pages: 1032-1036(2007)
- 作者机构：
  
  1. 重庆医科大学附属第一医院神经外科
  2. 重庆医科大学基础医学院组织学与胚胎学教研室
  3. 重庆医科大学附属第二医院肺科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2007，
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[1]徐丹,彭彦,王导新.融合自杀基因AdCD-UPRT对人肺癌细胞A_(549)的体外作用[J].重庆医科大学学报,2007(10):1032-1036.

XU Dan, et al. Study on AdCD-UPRT suicide gene mediated on lung cancer A₅₄₉ in vitro. [J]. Journal of Chongqing Medical University (10):1032-1036(2007)
[1]徐丹,彭彦,王导新.融合自杀基因AdCD-UPRT对人肺癌细胞A_(549)的体外作用[J].重庆医科大学学报,2007(10):1032-1036. DOI：

XU Dan, et al. Study on AdCD-UPRT suicide gene mediated on lung cancer A₅₄₉ in vitro. [J]. Journal of Chongqing Medical University (10):1032-1036(2007) DOI：

摘要

目的:研究融合自杀基因CD-UPRT系统对A549细胞的体外作用。方法:包装、扩增携带重组腺病毒AdCD-UPRT

并测定其滴度。检测AdCD-UPRT感染A549细胞的效率、毒性及目的基因的表达。用MTT法观察AdCD-UPRT/5-FC系统对A549细胞的体外抑制作用及旁观者效应。运用AO-EB染色、电镜及FCM观察AdCD-UPRT/5-FC系统对A549细胞的诱导凋亡作用。结果:CD-UPRT融合自杀基因对A549细胞显示出明显的生长抑制作用并存在旁观者效应

使细胞周期阻滞于S期并诱导细胞凋亡。结论:CD-UPRT融合自杀基因系统对A549细胞有较强的细胞毒作用。

Abstract

Objective:To study combination AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro. Methods:The recombinant AdCD-UPRT adenovirus was packaged and generated

and the title of virus was measured. The efficiency of transfecting target cells was detected

the toxicity of virus was measured

and the expression of CD-UPRT gene in A549 cells was assayed. The cytotoxicity and the bystander effect on A549 cells were measured by MTT assays respectively. The apoptosis was analyzed by AO-EB fluorescent staining

transmission electron microscope and flow cytometry. Results:The combination AdCD-UPRT had obviously growth inhibition effect on A549 cell and bystander effect. The cells were blocked in S phase and induced into apoptosis. Conclusion:The combination AdCD-UPRT suicide gene had cytotoxicity on lung cancer A549 in vitro.

关键词

腺病毒自杀基因肺肿瘤基因治疗

Keywords

adenovirussuicide genelung neoplasmsgene therapy

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Related Institution

Department of Biochemical Pharmacy,College of Pharmacy,the Second Military Medical University

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Department of Infectious Diseases,the Second Affiliated Hospital,Chongqing Medical University

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Study on AdCD-UPRT suicide gene mediated on lung cancer A549 in vitro

DOI：

摘要

Abstract

关键词

Keywords

references

Study on AdCD-UPRT suicide gene mediated on lung cancer A₅₄₉ in vitro