Research on the mechanism of sodium selenite inducing K562 cells apoptosis

SUN Yi; et al

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Research on the mechanism of sodium selenite inducing K562 cells apoptosis

更新时间：2024-01-17

- Research on the mechanism of sodium selenite inducing K562 cells apoptosis
- Journal of Chongqing Medical University Issue 6, Pages: 588-591(2007)
- 作者机构：
  
  1. 重庆医科大学医学检验系临床检验诊断学省部共建教育部重点实验室重庆市重点实验室
  2. 成都市第五人民医院检验科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R96
- Published：2007，
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[1]孙毅,阳强,黄琳,卢贤瑜.亚硒酸钠诱导K562细胞凋亡作用及机制探讨[J].重庆医科大学学报,2007(06):588-591.

SUN Yi, et al. Research on the mechanism of sodium selenite inducing K562 cells apoptosis. [J]. Journal of Chongqing Medical University (6):588-591(2007)
[1]孙毅,阳强,黄琳,卢贤瑜.亚硒酸钠诱导K562细胞凋亡作用及机制探讨[J].重庆医科大学学报,2007(06):588-591. DOI：

SUN Yi, et al. Research on the mechanism of sodium selenite inducing K562 cells apoptosis. [J]. Journal of Chongqing Medical University (6):588-591(2007) DOI：

摘要

目的:探讨亚硒酸钠诱导K562细胞凋亡的作用及机制。方法:不同浓度亚硒酸钠作用K562细胞后

MTT法检测细胞增殖抑制

电镜和TUNEL法检测K562细胞的凋亡

RT-PCR检测Bcl-2和Bax的mRNA表达变化

分光光度法检测Caspase-3的活性变化。结果:亚硒酸钠能抑制K562细胞的生长增殖

诱导其凋亡。20μmol/L亚硒酸钠作用K562细胞48h后

Bcl-2表达明显降低

Bax表达明显升高

Caspase-3活性升高

与对照组相比具有显著的统计学意义（P<0.01）。结论:亚硒酸钠能够诱导K562细胞凋亡

其机制可能与下调Bcl-2

上调Bax

活化Caspase-3有关。

Abstract

Objective:To investigate the effects of sodium selenite on the cell apoptosis of K562 cells and to elucidate its molecular mechanisms.Methods:K562 cells were treated with various concentrations of sodium selenite at different time points

and then MTT assay was employed to evaluate the effects of sodium selenite on the proliferative inhibition of K562 cells.MTT assay was employed to evaluate the effects of sodium selenite on the proliferative inhibition of K562 cells.Electronmicroscopy

and TUNEL were performed to confirm the apoptosis of K562 cells

RT-PCR was employed to analyze the mRNA expression levels of Bcl-2 and Bax.Colorimetric method were used to measure the activities of caspase-3 of K562.Results:Sodium selenite could inhibit proliferation of K562 cells and induce them to undergo apoptosis.RT-PCR results showed that sodium selenite could decrease the mRNA expression level of Bcl-2 and increase the level of Bax of K562 cells which had been treated with sodium selenite for 48h significantly

and the activity of caspase-3 elevated remarkably too.Compared with the control group

the expression levels of Bcl-2

Bax and acivity of caspase-3 in 20μmol/L sodium selenite treatment group were markedly changed(P<0.05).Conclusions:Sodium selenite can induce K562 cells to undergo apoptosis.The decrease of mRNA expression level of Bcl-2

the increase of Bax and activity of caspase-3 may be its mechanisms.

关键词

亚硒酸钠K562凋亡

Keywords

Sodium seleniteK562apoptosis

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