Immune efficacy of Ag85B DNA/H37Ra prime-boost vaccination regimen for tuberculosis in mice

WANG Jintong; et al

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Immune efficacy of Ag85B DNA/H37Ra prime-boost vaccination regimen for tuberculosis in mice

更新时间：2024-01-17

- Immune efficacy of Ag85B DNA/H37Ra prime-boost vaccination regimen for tuberculosis in mice
- Journal of Chongqing Medical University Issue 6, Pages: 584-587(2007)
- 作者机构：
  
  1. 重庆医科大学临床免疫学教研室临床检验诊断学省部共建教育部重点实验室
  2. 重庆医科大学临床免疫学教研室临床检验诊断学省部共建教育部重点实验室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2007，
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[1]王锦彤,卢贤瑜,冉柏林.Ag85B DNA/H37Ra序贯免疫效果的探讨[J].重庆医科大学学报,2007(06):584-587.

WANG Jintong, et al. Immune efficacy of Ag85B DNA/H37Ra prime-boost vaccination regimen for tuberculosis in mice. [J]. Journal of Chongqing Medical University (6):584-587(2007)
[1]王锦彤,卢贤瑜,冉柏林.Ag85B DNA/H37Ra序贯免疫效果的探讨[J].重庆医科大学学报,2007(06):584-587. DOI：

WANG Jintong, et al. Immune efficacy of Ag85B DNA/H37Ra prime-boost vaccination regimen for tuberculosis in mice. [J]. Journal of Chongqing Medical University (6):584-587(2007) DOI：

摘要

目的:初步探讨含结核分枝杆菌Ag85B成熟蛋白的DNA（pTB30m）和结核菌H37Ra序贯免疫小鼠产生的特异性免疫应答。方法:重组质粒pTB30m用碱裂解法制备后进行质量鉴定。用pTB30m肌注初次免疫小鼠2周后

H37Ra皮内加强免疫作为DNA-85B/H37Ra组

同时设定DNA-85B/BCG组、H37Ra组、BCG组及未免疫组。免疫4周或8周后

用ELISA法检测小鼠血清中抗PPD IgG抗体的水平和MTT法检测其脾淋巴细胞的刺激指数（SI）。结果:酶切鉴定pTB30m所含外源基因片段大小正确

并且纯度较高。DNA-85B/H37Ra组小鼠血清中抗PPD IgG水平、脾淋巴细胞的SI均显著高于未免疫组（P<0.05）;其抗PPD IgG水平稍高于DNA-85B/BCG组、H37Ra组及BCG组

但它们之间无显著性差异（P>0.05）;其脾淋巴细胞的SI显著高于H37Ra、BCG组（P<0.05）

而与DNA-85B/BCG组比较

仅在4周有显著性差异。在DNA-85B/H37Ra组内

SI在4周显著高于8周（P<0.05）

而血清中抗PPD IgG水平8周均显著高于4周（P<0.05）。结论:DNA-85B/H37Ra序贯免疫策略可以诱导小鼠产生特异性体液免疫和细胞免疫

初步证明其免疫效果略优于BCG。

Abstract

Objective:To investigate the immune efficacy after sequential immunization with Mycobacterium tuberculosis DNA vaccine encoding mature form of Ag85B(pTB30m)and Mycobacterium tuberculosis H37Ra in mice.Methods:Much of highly pure plasmid DNA(pTB30m)extracted by alkaline lysis method was confirmed by restriction endonuclease digestion.Then

its DNA concentration and purity were determined by UV spectrophotometry.At various intervals(4weeks

8weeks)after sequential immunization

ELISA was used to detect the level of the serum antibody against PPD.Also

the spleen lymphocytes of mice were cultured with PPD in vitro.Lymphocyte transformation was detected by MTT assay.Results:Prepared pTB30m was highly pure and came to the needed concentration.Compared with Group Naive control

the specific antibody levels against PPD and the stimulation index(SI)of spleen lymphocytes were all statistically higher in Group DNA-85B/H37Ra(P<0.05)

but the specific antibody levels were not significant different(P>0.05)

as compared with Group DNA-85B/BCG

Group H37Ra and Group BCG

However

compared with Group H37Ra and Group BCG

the SI of mice was significantly larger in Group DNA-85B/H37Ra(P<0.05)

but compared with Group DNA-85B/BCG

it was only statistically higher at 4weeks but not at 8 weeks.Simultaneously

in Group DNA-85B/H37Ra

the SI of the spleen lymphocytes was much higher at 4weeks than that at 8weeks(P<0.05)

but the specific antibody levels were much higer at 8weeks than those at 4weeks(P<0.05).Conclusion:The DNA-85B/H37Ra regimen for tuberculosis prevention in mice stimulate the specific antibody and induce cellular immunity.The immune efficacy of the DNA-85B/H37Ra regimen is slighterly better than that of BCG.

关键词

结核分枝杆菌序贯免疫策略初免-加强免疫接种策略抗原Ag85BH37Ra

Keywords

mycobacterium tuberculosisSequential immunization strategyPrime-boost vaccination regimenAntigen 85BH37Ra

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