Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell

XU Xiong-yang; et al

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Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell

更新时间：2024-01-17

- Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell
- Journal of Chongqing Medical University Issue 4, Pages: 411-415(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院消化内科
  2. 重庆医科大学附属第二医院消化内科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.7
- Published：2008，
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[1]胥雄阳,何松.苦参碱逆转人肝癌细胞株QGY/CDDP多药耐药性的实验研究[J].重庆医科大学学报,2008(04):411-415.

XU Xiong-yang, et al. Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell. [J]. Journal of Chongqing Medical University (4):411-415(2008)
[1]胥雄阳,何松.苦参碱逆转人肝癌细胞株QGY/CDDP多药耐药性的实验研究[J].重庆医科大学学报,2008(04):411-415. DOI：

XU Xiong-yang, et al. Experimental study of matrine to reverse drug-resistance of human multidrug-resistance hepatocellular carcinoma cell. [J]. Journal of Chongqing Medical University (4):411-415(2008) DOI：

摘要

目的:探讨中药苦参碱（Mat）对QGY人肝癌细胞及其耐药细胞株QGY/CDDP的细胞生物学作用和可能的耐药逆转作用机制。方法:MTT法检测苦参碱对两细胞的半数抑制浓度（IC50值）并培养Mat作用的QGY/CDDP细胞。比较3组细胞在细胞生长曲线、倍增时间、细胞周期、化疗药物耐药及逆转和细胞内CDDP含量的差别。免疫细胞化学法测定3组细胞P-gp、MRP、Bax蛋白的表达。结果:苦参碱可抑制QGY及其耐药细胞株QGY/CDDP的生长

无交叉耐药性。Mat作用的QGY/CDDP细胞与QGY及其耐药细胞株QGY/CDDP在生物学特性方面表现出一定的差异

细胞的P-gp、MRP、Bax蛋白表达发生变化

降低QGY/CDDP细胞P-gp、MRP蛋白表达

增加了Bax蛋白的表达

增强CDDP对QGY/CDDP细胞的毒性

其逆转耐药倍数为1.9倍。结论:低毒剂量的苦参碱可增强CDDP对QGY/CDDP细胞的毒性

具有部分逆转人肝癌耐药细胞QGY/CDDP的耐药作用。

Abstract

Objective:To investigate the biologic roles and potential reverse mechanisms of matrine in human hepatocellular carcinoma cell line QGY and QGY/CDDP.Methods:The MTT assay was used to detect the IC50s of QGY and QGY/CDDP cells

and QGY/CDDP cell were cultivated with matrine.The distinctions of three groups were compared in cell’s appearance

growth curve

doubling time

cell cycle

drug-resistance of chemotherapeutics and CDDP contents of intra-cells.Immunocytochemical method was applied to detect the expression of P-gp

MRP and Bax.Results: Matrine could inhibit the growth of QGY and QGY/CDDP cells with no cross drug-resistance.There were some biological differences among QGY

QGY/CDDP and Mat-QGY/CDDP cells.The expressions of P-gp and MRP were decreased;Bax expression was increased

CDDP’s toxic effect on QGY/CDDP cells was enhanced

and the drug sensitivity was elevated in Mat-QGY/CDDP cells.Conclusion:Matrine at lower toxic-dose could enhance the toxicity of CDDP on QGY/CDDP cells

and partly effective in reversing the drug-resistance in human multidrug-resistant hepatocellular carcinoma QGY/CDDP cells.

关键词

肝癌细胞多药耐药性苦参碱

Keywords

Hepatocellular carcinomamultidrug resistanceMatrine

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