Efficacy comparison of three primary cell culture methods for mature rat adipocytes

ZHANG Yan; et al

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Efficacy comparison of three primary cell culture methods for mature rat adipocytes

更新时间：2024-01-17

- Efficacy comparison of three primary cell culture methods for mature rat adipocytes
- Journal of Chongqing Medical University Issue 1, Pages: 69-72(2008)
- 作者机构：
  
  1. 重庆医科大学附属儿童医院儿保科
  2. 重庆医科大学附属儿童医院儿保科,重庆,400014
- 作者简介：
- 基金信息：
- DOI：
  CLC： R329
- Published：2008，
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[1]张艳,张晓萍,刘官信,刘友学.大鼠成熟脂肪细胞原代培养方法比较[J].重庆医科大学学报,2008(01):69-72.

ZHANG Yan, et al. Efficacy comparison of three primary cell culture methods for mature rat adipocytes. [J]. Journal of Chongqing Medical University (1):69-72(2008)
[1]张艳,张晓萍,刘官信,刘友学.大鼠成熟脂肪细胞原代培养方法比较[J].重庆医科大学学报,2008(01):69-72. DOI：

ZHANG Yan, et al. Efficacy comparison of three primary cell culture methods for mature rat adipocytes. [J]. Journal of Chongqing Medical University (1):69-72(2008) DOI：

摘要

目的:探寻体外分离培养大鼠成熟脂肪细胞的最佳方法。方法:采用器官培养法、天花板培养法和改良天花板培养法3种不同的原代培养方法

体外培养大鼠成熟脂肪细胞。通过观察显微镜下细胞形态及油红O染色结果

比较3种培养方法所培养的脂肪细胞的差异。结果:3种培养方法均培养出成熟的脂肪细胞

细胞形态一致

其中改良天花板培养法的培养效率最高

培养出的细胞形态最好。结论:改良天花板培养方法是一种长期稳定的大鼠成熟脂肪细胞原代培养方法

为进行脂肪细胞的相关研究提供良好的技术平台。

Abstract

Objective:To identify the best method for isolating and culturing mature rat adipocytes in vitro.Methods:Three different primary culture methods

i.e.organ culture

ceiling culture

and modified ceiling culture were used in the present study.Mature Sprague-Dawley rat adipocytes were cultured in vitro.The cells were observed under microscope and oil red O staining to compare the distinction of cells with different culture methods in the same condition.Rusults:Adipocytes were successfully cultured by the three primary cell culture methods.Cells cultured were highly homogenous.Modified ceiling culture method was the best in terms of the efficiency of culturing and cell morphology.Conclusion:Modified ceiling culture

is a long-term stable primary culture method for mature rat adipocyte

providing a good technique platform for the studies related to adipocytes.

关键词

脂肪细胞细胞培养原代培养大鼠

Keywords

adipocytescell cultureprimary culturerats

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