Objective:To investigate the rapid establishment of nude mice-C6 glioma model and its application feasibility in research experiments.Methods:C6 mice glioma cell-suspension was inolulated to the male and female nude mice subcutaneously.The period for tumor tissue to grow out was detected and the histological and immunohistochemical features of tumor were observed.Tumor tissue was used to do allograft transplant for the male and female nude subcutaneously.The sprouted time of subcutaneous transplant tumor in nude mice was detected.Pathological and immunohistochemical features of tumor were studied.Results:(1)Success rates of both two methods were 100%

tumor grew in good condition

and the histological and immunohistochemical examination results of tumor were consistent with glioma cell morphology;(2)The method of cell inoculation to establish model took a long time.Tumor growing speeds in male and female nude mice were different and the difference had statistical significance(P<0.05);(3)Tumor tissue allograft transplant took shorter timer.Tumor growing speeds in male and female basically the same

without statistical significant difference(P>0.05) Conclusion:(1)Tumor tissue transplant can establish subcutaneous xenograft model quickly

and to provide the reliable animal model for studying of glioma、biological characteristics and gene therapy;(2)Tumor tissue allograft can shorten the establishment time of animal models saving the cost of scientific research.