Comparison of two experimental ulcerative colitis models in rat

DUAN Zheng; et al

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Comparison of two experimental ulcerative colitis models in rat

更新时间：2024-01-17

- Comparison of two experimental ulcerative colitis models in rat
- Journal of Chongqing Medical University Issue 1, Pages: 66-68(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院组织胚胎学教研室
  2. 重庆医科大学基础医学院组织胚胎学教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R-332;R574.62
- Published：2008，
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[1]段征,汪维伟,姜蓉.两种溃疡性结肠炎大鼠模型的比较[J].重庆医科大学学报,2008(01):66-68+72.

DUAN Zheng, et al. Comparison of two experimental ulcerative colitis models in rat. [J]. Journal of Chongqing Medical University (1):66-68(2008)
[1]段征,汪维伟,姜蓉.两种溃疡性结肠炎大鼠模型的比较[J].重庆医科大学学报,2008(01):66-68+72. DOI：

DUAN Zheng, et al. Comparison of two experimental ulcerative colitis models in rat. [J]. Journal of Chongqing Medical University (1):66-68(2008) DOI：

摘要

目的:比较2

6-三硝基苯磺酸（2

6-trinitro-benzene-sulfonic acid

TNBS）与免疫复合法介导的溃疡性结肠炎（Ulcerative colitis

UC）动物模型的效果。方法:SD大鼠随机分为免疫复合法（Ⅰ组）、TNBS/乙醇（Ⅱ组）、乙醇对照（Ⅲ组）和生理盐水对照（Ⅳ组）。Ⅰ组先间隔2周给予兔肠黏膜制备的抗原乳化液（含抗原8 mg）免疫2次。4组均于禁食24h后用0.65ml不同液体灌肠:Ⅰ、Ⅱ组均为100mg TNBS/kg+50%乙醇;Ⅲ组为50%乙醇;Ⅳ组为生理盐水。造模后第1、21d

第8周、12周处死大鼠

观察大体病灶及光镜下组织形态学改变。结果:Ⅰ、Ⅱ组均出现糜烂、溃疡等典型UC结肠病变

Ⅰ组还伴有末段回肠病变;Ⅰ组的病变可维持8周

Ⅱ组病变3周后趋向愈合。Ⅲ组病变仅黏膜充血水肿和少量糜烂或浅表溃疡

第21d痊愈。Ⅳ组未见组织损伤。结论:免疫复合法重复性好

病变更类似人UC

持续时间长

是较理想的造模方法。

Abstract

Objective:To compare the rat models of ulcerative colitis induced by 2

6-trinitro-benzene-sulfonic acid(TNBS) and immune-comb method.Methods:SD rats were randomly distributed into groupⅠ(immune-comb method)

groupⅡ(TNBS/ethanol)

groupⅢ(50% ethanol) and groupⅣ(control).The rats in groupⅠwere sensitized 2 times by antigen made of rabbit colon mucosa in 2-week intervals.After abrosia for 24 hours

each rat in four groups received enema of 0.65 ml different fluids

which were:TNBS 100mg/kg +50%ethanol for groupⅠand Ⅱ

50%ethanol for group Ⅲ

and normal sodium for control group.Rats were sacrificed on day 1

21 and week 8

12 after enema to observe the macroscopical focus of infection in bowels and the microscopical changes.Results:The typical erosion and ulcer changes appeared in groupⅠandⅡ

and there was accompanying pathological changes in the end piece of ileum in groupⅠ.The pathological changes for groupⅠcould keep for 8 weeks

while those for group Ⅱtrended to heal 3 weeks later.The pathological changes in groupⅢonly were hyperemia

oedema and a few anabrosis or superficial ulcer

keeped for 21days.There was no tissue damage in group Ⅳ.Conclusion:Immune-comb method is comparatively the ideal method to establish the model

because it has a good reproducibility

a long persistence time

and the pathological changes resemble more to those of man..

关键词

溃疡性结肠炎模型大鼠

Keywords

ulcerative colitismodelrat

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