The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line

LIU Xiao; et al

您当前的位置：

首页 >

文章列表页 >

The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line

更新时间：2024-01-17

- The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line
- Journal of Chongqing Medical University Issue 1, Pages: 25-28(2008)
- 作者机构：
  
  1. 重庆医科大学附属第二医院呼吸内科
  2. 重庆医科大学附属第二医院呼吸内科,重庆,400010
  3. 重庆医科大学基础医学研究所
  4. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2008，
扫描看全文
[1]刘晓,蒋幼凡,岳秀,雷淑慧.X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响[J].重庆医科大学学报,2008(01):25-28.

LIU Xiao, et al. The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line. [J]. Journal of Chongqing Medical University (1):25-28(2008)
[1]刘晓,蒋幼凡,岳秀,雷淑慧.X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响[J].重庆医科大学学报,2008(01):25-28. DOI：

LIU Xiao, et al. The effect of X-irradiation on adhesion molecules and their related biological characteristics of A549 human lung adenocarcinoma cell line. [J]. Journal of Chongqing Medical University (1):25-28(2008) DOI：

摘要

目的:探讨X射线对肺腺癌细胞粘附分子及其相关生物学特性的影响。方法:不同剂量X射线照射肺腺癌细胞A549

MTT法检测细胞增殖抑制;免疫细胞化学法蛋白水平检测细胞中E-cadherin及其连环蛋白β-catenin的表达;Transwell小室检测细胞的侵袭力。结果:X射线对A549细胞的增殖抑制作用呈剂量依赖性;照射后E-cadherin和β-catenin蛋白的表达均显著高于对照组（P<0.05）

其变化趋势是随照射剂量增加表达逐渐增加;Transwell小室结果表明随着照射剂量的增加

细胞侵袭力逐渐降低（P<0.05）。结论:X射线上调A549细胞上粘附分子E-cadherin和β-catenin在细胞中的表达

增加细胞间的粘附力

降低肿瘤细胞的侵袭力

这可能是X射线抑制肺腺癌细胞转移的作用机制之一。

Abstract

Objective:To investigate the effect of X-irradiation on Adhesion molecules and their related biological characteristics of Human Lung.Methods:A549 cells were irradiated with varying doses of X-rays.MTT assay was employed to evaluate the effects of X-irradiation on the proliferative inhibition of A549 cells.The immunocytochemical method was employed to analyze the protein expression levels of E-cadherin and β-catenin.The transwell chambers were employed to evaluate the invasion of A549 cells.Results:X-irradiation could inhibit the proliferation of A549 cells in a dose-dependent manner.The protein expression of E-cadherin and β-catenin in A549 were higher than that in control group(P<0.05).And the cell invasions were lower than that in control group(P<0.05).The expression of E-cadherin

β-catenin and cell invasions showed a direct correlation with the dose.Conclusion:Up-regulating the expression of E-cadherin and β-catenin on A549 cells

which enhanced the adhesion between cells and depressed the invasion.That may be the mechanism of X-irradiation inhibiting the metastasis of Human Lung Adenocarcinoma Cell line.

关键词

X射线A549E-cadherinβ-catenin转移

Keywords

X-irradiationA549E-cadherinβ-cateninmetastasis

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Molecular mechanism of Buzhong Yiqi Decoction in coordination with Siβ-catenin in intervention against cisplatin resistance in lung adenocarcinoma by regulating the Wnt/β-catenin signaling pathway

Screening of differentially expressed proteins related to metastasis in hepatocellular carcinoma based on iTRAQ

Research progress in the melanomas after nanosecond pulsed electric fields treatment

Hepatitis C virus core protein inhibits the E-cadherin promoter activity

Expression of metabolic pathway in A549 endotracheal orthotopic transplantation lung cancer model

Related Author

Li He

Mu Qirui

Wang Zhe

Wang Ying

Yu Dan

Gao Yuan

Song Linlan

Yang Min

Related Institution

Department of Pathology，College of Integrative Medicine，Liaoning University of Traditional Chinese Medicine

Institute for Viral Hepatitis

Department of Oral Medicine,School of Stomatology,Lanzhou University

Key Laboratory of Molecular Biology on Infectious Diseases Founded by the Ministry of Education,Chongqing Medical University

School of Public Health and Management,Research Center for Medicine and Social Development,Innovation Center for Social Risk,Chongqing Medical University

⁰