Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell

LIU Yong-quan; et al

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Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell

更新时间：2024-01-17

- Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell
- Journal of Chongqing Medical University Issue 2, Pages: 140-143(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院呼吸内科
  2. 重庆医科大学附属第一医院呼吸内科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R734.2
- Published：2008，
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[1]刘永全,江涛,冯燕梅.脂质体转染survivin siRNA对A549细胞增殖及凋亡的影响[J].重庆医科大学学报,2008(02):140-143.

LIU Yong-quan, et al. Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell. [J]. Journal of Chongqing Medical University (2):140-143(2008)
[1]刘永全,江涛,冯燕梅.脂质体转染survivin siRNA对A549细胞增殖及凋亡的影响[J].重庆医科大学学报,2008(02):140-143. DOI：

LIU Yong-quan, et al. Effect of liposomal transfection of survivin siRNA on proliferation and apoptosis of A549 cell. [J]. Journal of Chongqing Medical University (2):140-143(2008) DOI：

摘要

目的:探讨脂质体转染survivin siRNA对人肺腺癌A549细胞增殖、凋亡的影响。方法:人工合成抑制survivin基因的siRNA片段

通过脂质体转染到A549细胞内

倒置荧光显微镜下观察细胞形态学变化;用四氮唑盐（MTT）法观察转染后24h

48h

72h对细胞增生的影响;流式细胞术检测各组细胞周期变化和凋亡指数;RT-PCR检测survivin mRNA的表达。结果:转染siRNA后的A549细胞

细胞增殖受到显著抑制

细胞明显出现凋亡

survivin基因mRNA表达显著下降。结论:应用RNA干扰靶向抑制survivin基因可以显著抑制A549细胞增殖

促进细胞凋亡。survivin可能成为肺癌基因治疗的一个新靶点。

Abstract

Objective:To explore the effect of liposomal transfection of survivin siRNA on the proliferation and apoptosis of lung adenocarcinoma cell lines A549.Method:Survivin siRNA was synthetized and transfected into A549 cell by liposome.Cell morphological change was observed with inverted fluorescent microscope

cell proliferation was examined by MTT assay at 24h 48h and 72h after transfection.cell proliferation and apoptotic index detected by flow cytometry and survivin mRNA expression determined by RT-PCR.Results:For the A549 cell transfected with siRNA cell proliferation was ihibited significantly

cell apoptosis appeared obvious and the expression of survivin mRNA significantly decreased.Conclusion:Survivin siRNA is able to inhibit the proliferation of lung adenocarcinoma cell lines A549 and induce the cell apoptosis.Survivin might become a new target for lung carcinoma gene therapy.

关键词

SurvivinsiRNA细胞凋亡肺癌RNAi

Keywords

SurvivinsiRNAcell apoptosisLung carcinomaRNAi

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Related Institution

Department of Pathology，College of Integrative Medicine，Liaoning University of Traditional Chinese Medicine

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Department of Otorhinolaryngology,Henan Medical College

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Key Laboratory of Laboratory Medical Diagnosis of Education Ministry,Department of Laboratory Medicine,Chongqing Medical University

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