Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines

CAI Li; et al

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Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines

更新时间：2024-01-17

- Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines
- Journal of Chongqing Medical University Issue 2, Pages: 136-139(2008)
- 作者机构：
  
  1. 重庆医科大学基础医学院病理教研室
  2. 重庆医科大学基础医学院病理教研室,重庆,400016
- 作者简介：
- 基金信息：
- DOI：
  CLC： R735.35
- Published：2008，
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[1]蔡莉,王娅兰,林晓.PARP抑制与小鼠结肠癌CT26细胞生长活性的关系[J].重庆医科大学学报,2008(02):136-139.

CAI Li, et al. Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines. [J]. Journal of Chongqing Medical University (2):136-139(2008)
[1]蔡莉,王娅兰,林晓.PARP抑制与小鼠结肠癌CT26细胞生长活性的关系[J].重庆医科大学学报,2008(02):136-139. DOI：

CAI Li, et al. Relationship between PARP inhibition and the growth activity ofmurine colon carcinoma CT26 cell lines. [J]. Journal of Chongqing Medical University (2):136-139(2008) DOI：

摘要

目的:探讨PARP抑制与结肠癌CT26细胞生长活性的关系。方法:采用PARP抑制剂5-氨基异喹啉酮（5-aminoisoquinolinone

5-AIQ）处理小鼠结肠癌CT26细胞

MTT法检测CT26细胞离体生长

免疫荧光双标、激光共聚焦检测结肠癌CT26细胞核内PARP与NF-κB p65的表达。结果:不同浓度的5-AIQ处理组（0.1mmol/L、0.5mmol/L、1.0mmol/L）结肠癌CT26细胞生长抑制率分别为17.52%、27.63%和39.93%

抑制率随5-AIQ浓度的增加而增加

差异具显著性（P<0.05）。PARP与NF-κB p65在CT26细胞核内存在共表达

且呈正相关（P<0.05）。5-AIQ处理组与5-AIQ未处理组相比

PARP与NF-κB p65的表达均减弱

差异具显著性（P<0.05）

PARP与NF-κB p65亦呈正相关（P<0.05）。结论:PARP抑制剂5-AIQ可以抑制结肠癌CT26细胞生长活性。其可能与PARP抑制

使NF-κB活性降低有关。PARP在结肠癌生长过程中可能具有重要作用。

Abstract

Objective:To investigate the relationship between poly(ADP-ribose)polymerase(PARP) inhibition and the growth activity of murine colon carcinoma CT26 cell lines in vitro.Methods:The murine colon carcinoma CT26 cell lines were treated with PARP inhibitior 5-AIQ in vitro.MTT assay was used to determine the growth activity of CT26 cells.The expressions of PARP and NF-κB p65 in the nuclei of CT26 cells were investigated by double labelling immunofluorescence and laser scanning cofocal microscope.Results:The inhibitory rates of the growth in 5-AIQ-treated colon carcinoma CT26 cell groups were 17.52%、27.63% and 39.93%

respectively.The inhibitory rate increased with the raising of the 5-AIQ concentration(0.1mmol/L

0.5mmol/L

and1.0mmol/L)

and the difference was significant(P<0.05).There was a co-expression and positive correlation of PARP and NF-κBp65 in the nuclei of colon carcinoma CT26 cells(P<0.05).The expressions of PARP and NF-κB p65 were attenuated in 5-AIQ-treated CT26 cell groups

and the correlation of PARP and NF-κB p65 was also positive(P<0.05).Conclusion:PARP inhibitior 5-AIQ can inhibit the growth activity of colon carcinoma CT26 cells in vitro

and this may be through inhibiting PARP to reduce the activity of NF-κB.PARP may play an important role in the proliferation of colon carcinoma.

关键词

PARP5-AIQ生长结肠癌

Keywords

PARP5-AIQgrowthcolon carcinoma

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