Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats

ZHENG Chuan-dong; et al

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Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats

更新时间：2024-01-17

- Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats
- Journal of Chongqing Medical University Issue 2, Pages: 144-146(2008)
- 作者机构：
  
  1. 重庆医科大学附属第一医院泌尿外科
  2. 重庆医科大学附属第一医院血管外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R692
- Published：2008，
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[1]郑传东,苟欣,武国,杨华安,蒲晓峰,何卫阳.雷帕霉素对肾缺血再灌注大鼠肾功和肾组织超微结构的影响[J].重庆医科大学学报,2008(02):144-146+151.

ZHENG Chuan-dong, et al. Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats. [J]. Journal of Chongqing Medical University (2):144-146(2008)
[1]郑传东,苟欣,武国,杨华安,蒲晓峰,何卫阳.雷帕霉素对肾缺血再灌注大鼠肾功和肾组织超微结构的影响[J].重庆医科大学学报,2008(02):144-146+151. DOI：

ZHENG Chuan-dong, et al. Effects of RPM on renal function and ultrastructure after ischemia-reperfusion in rats. [J]. Journal of Chongqing Medical University (2):144-146(2008) DOI：

摘要

目的:探讨雷帕霉素（Rapamycin

RPM）在大鼠肾缺血再灌注损伤时肾功能和肾组织超微结构改变中的作用。方法:30只雄性Wistar大鼠随机分为3组:假手术组（切除右肾

分离左肾动脉

不阻断血流

灌胃给等量生理盐水）、手术组（切除右肾

分离左肾动脉

阻断血流

缺血前灌胃给等量生理盐水）、药物组(切除右肾

分离左肾动脉

阻断血流

缺血前灌胃给RPM（4mg/（kg.d）×3d

最后一次术前2h灌胃）。大鼠急性缺血性肾损伤模型为切除右肾

分离左肾动脉

阻断血流45min

再灌注24h

观察肾功能和肾组织超微结构改变。结果:肾缺血再灌注组血肌酐（168±37）μmol/L、血尿素氮（22±6）mmol/L

雷帕霉素组血肌酐（113±17）μmol/L、血尿素氮（13.8±2.3）mmol/L;2组比较差异有统计学意义（P<0.05）。电镜:假手术组肾小管上皮细胞超微结构正常;手术组肾小管管腔被大量坏死脱落细胞碎片堵塞

上皮细胞胞浆内脂滴堆积

细胞有凋亡迹象;药物组肾小管超微结构损害轻微。结论:雷帕霉素对大鼠肾缺血再灌注损伤时肾功能和肾组织超微结构有明显保护作用。

Abstract

Objective:To investigate the effect of rapamycin on renal function and ultrastructure changes after renal ischemia-reperfusion in rats.Methods:Acute ischemic renal injury model was established(right kidney ectomized

45 min of left renal ischemia and reperfusion for 24h).Thirty male Wistar rats were randomly divided into 3 groups:sham operation group(control group or group C

without renal ischemia)

renal ischemia-reperfusion group(ischemia-reperfusion group or group I

without rapamycin)

renal ischemia-reperfusion and(RPM 4mg/(kg·d)×3d

reperfusion at 2h before the last operation) intravenous injection group(rapamycin group or group U).changes of renal function and renal uhrastructure were measured BUN level

serum creatinine values.Results:Serum creatinine(168±37)μmol/L and BUN concentration(22±6)mmol/L in group I were significantly higher than those in group U:serum creatinine(113±17)μmol/L and BUN concentration(13.8±2.3)mmol/L(P<0.05).under the observation of electromicrosope

the renal ultrastructure of group Cwas normal

the ultrastructure was greatly injured in group I

large quantity of apoptosis residues were stopped in the renule intracellular lipid accumulation and cell apoptotis presented meanwhile

it was obviously ameliorated in group U.Conclusion:Rapamycin greatly improves renal function and provides remarkable protection on renal ultrastructure after ischemia-reperfusion of kidney in rats.

关键词

再灌注损伤超微结构雷帕霉素

Keywords

reperfusion injuryUhrastructureRapamycin(RPM)

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