Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-γ

YANG Qi-hong; et al

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Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-γ

更新时间：2024-01-17

- Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-γ
- Journal of Chongqing Medical University Issue 2, Pages: 147-151(2008)
- 作者机构：
  
  1. 第三军医大学西南医院老年科
  2. 中国人民解放军第113医院老年科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R587.2
- Published：2008，
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[1]杨启红,徐强,张红,司良毅.糖基化终产物抑制THP-1巨噬细胞过氧化物酶体增殖物激活受体γ的表达[J].重庆医科大学学报,2008(02):147-151.

YANG Qi-hong, et al. Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-γ. [J]. Journal of Chongqing Medical University (2):147-151(2008)
[1]杨启红,徐强,张红,司良毅.糖基化终产物抑制THP-1巨噬细胞过氧化物酶体增殖物激活受体γ的表达[J].重庆医科大学学报,2008(02):147-151. DOI：

YANG Qi-hong, et al. Advanced glycation end-products decreases THP-1 macrophages expression of peroxisome proliferator activated receptor-γ. [J]. Journal of Chongqing Medical University (2):147-151(2008) DOI：

摘要

目的:观察糖基化终产物（AGEs）对THP-1巨噬细胞过氧化物酶体增殖物激活受体γ（PPARγ）表达的影响

探讨AGEs在糖尿病动脉粥样硬化中可能的作用。方法:用佛波酯（PMA）诱导THP-1单核细胞72h使其分化为巨噬细胞

并将糖基化-牛血清白蛋白（AGE-BSA）与巨噬细胞共同孵育

运用RT-PCR和免疫细胞化学法分别检测巨噬细胞PPARγmRNA和蛋白的表达水平。结果:PMA诱导72h后

THP-1细胞停止增殖

由单核细胞分化成为巨噬细胞。50、100、200和400μg/mlAGE-BSA处理24h后

巨噬细胞PPARγmRNA相对表达量分别是1.235±0.044、0.752±0.055、0.494±0.026、0.277±0.025;PPARγ蛋白的平均积分光密度值分别为36.460±0.625、24.561±0.636、19.326±0.803、12.715±0.752

二者较100μg/ml牛血清白蛋白（BSA）组均明显降低（P<0.05）。200μg/ml AGE-BSA作用12、24、36和48h后

巨噬细胞PPARγmRNA相对表达量分别是1.564±0.060、1.260±0.043、0.467±0.033、0.360±0.012;PPARγ蛋白的平均积分光密度值分别为32.502±0.739、22.234±0.835、16.568±0.683、11.537±0.547

二者较0h组也明显降低（P<0.05）。结论:AGEs可下调THP-1巨噬细胞PPARγmRNA和蛋白的表达水平

且呈浓度和时间依赖性。

Abstract

Objective:To investigate the effect of advanced glycosylation end-products(AGEs) on the expression of peroxisome proliferator-activated receptor-γ(PPARγ) in THP-1 macrophages and explore the function of AGEs in atherosclerosis.Methods: Differentiation of THP-1 cell was induced by PMA.The THP-1 macrophages were exposed to AGE-modified bovine serum albumin(AGE-BSA) in different concentration or the same concentration for different time.Expression of PPARγ mRNA and protein in THP-1 macrophages was measured by RT-PCR and immunocytochemical method.Results:RT-PCR showed that expression of PPARγ mRNA was 1.235±0.044

0.752±0.055

0.494±0.026

0.277±0.025 in 50

100

200 and 400μg/ml AGE-BSA groups.PPARγ mRNA expression in THP-1 macrophages following 200μg/ml AGE-BSA for 12

48h was 1.260±0.043

0.467±0.033

0.360±0.012.Western-Blot showed that after exposure of THP-1 macrophages to 50

100

200and 400mg/L AGE-BSA

the average integrated optical density values of PPARγ protein expression were 36.460±0.625

24.561±0.636

19.326±0.803

12.715±0.752 respetively

significantly lower than that in BSA group(P<0.05)

the average integrated optical density values of PPARγ protein expression in THP-1macrophages following 200mg/L AGE-BSA for 12

48h were 32.502±0.739

22.234±0.835

16.568±0.683

11.537±0.547

lower than that in 0h group(P<0.05).Conclusion:AGE-BSA could decrease the expression levels of mRNA and proteins for PPARγ in THP-1 macrophages in a dose-and time-dependent manner

which might be partly involved in atherogenesis in diabetic patients.

关键词

糖基化终产物巨噬细胞过氧化物酶体增殖物激活受体γ基因表达动脉粥样硬化

Keywords

Advanced Glycation End ProductsMacrophagesPeroxisome Proliferator Activated Receptor γgene expressionatherosclerosis

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