Empirical study on application of insulin in treating traumatic spinal cord injury in rat models

JANG Wei; et al

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Empirical study on application of insulin in treating traumatic spinal cord injury in rat models

更新时间：2024-01-17

- Empirical study on application of insulin in treating traumatic spinal cord injury in rat models
- Journal of Chongqing Medical University Issue 9, Pages: 1103-1107(2008)
- 作者机构：
  
  重庆医科大学附属第一医院骨科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R651.2
- Published：2008，
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[1]江伟,蒋电明.胰岛素在大鼠脊髓损伤治疗中的实验研究[J].重庆医科大学学报,2008(09):1103-1107.

JANG Wei, et al. Empirical study on application of insulin in treating traumatic spinal cord injury in rat models. [J]. Journal of Chongqing Medical University (9):1103-1107(2008)
[1]江伟,蒋电明.胰岛素在大鼠脊髓损伤治疗中的实验研究[J].重庆医科大学学报,2008(09):1103-1107. DOI：

JANG Wei, et al. Empirical study on application of insulin in treating traumatic spinal cord injury in rat models. [J]. Journal of Chongqing Medical University (9):1103-1107(2008) DOI：

摘要

目的:观察胰岛素对急性脊髓损伤是否有保护作用及其可能的机理。方法:60只SD大鼠

雌雄不限

随机分为A组（损伤对照组）和B组（胰岛素组）

每组各30只大鼠

Allen法建立脊髓损伤模型

B组给与1IU/kg的胰岛素

1日3次腹腔灌注

连续7d

A组给与等量同次数的生理盐水腹腔灌注

在伤后8h、第3d、第7d、第14d、第28d观察脊髓凋亡细胞

在第1d、第3d、第7d、第14d、第28d观察尼氏体和神经元数量、GAP-43阳性细胞、BBB评分。结果:B组神经元数量、GAP-43阳性细胞、BBB评分在多个时相点要好于A组（P<0.05或P<0.01）

凋亡细胞B组明显少于A组（P<0.05或P<0.01）。结论:胰岛素能通过抑制脊髓神经细胞的凋亡坏死、促进神经轴突的修复和再生来达到保护脊髓神经细胞、促进功能恢复的目的。

Abstract

Objective:To observe neuroprotective effects of insulin on traumatic spinal cord injury in rat models and discuss its potential mechanisms. Methods:60 SD rats were randomly divided into A group(control group) and B group(insulin group)

with 30 rats in each group

The animal SCI model was established according to method Allen’s

A group and B group were respectively infused with isotonic Na chloride or 1Iu/kg insulin through abdominal cavity

three times a day

for 7 days. apoptosis neurons were observed on 8 hours

3 days

7 days

14 days

and 28 days after SCI

and tigroid body

neurons

GAP-43 positive cells

and BBB score were measured on 1 day

3 days

7 days

14 days

and 28 days after SCI. Results:Neuron number

GAP-43 positive cells

and BBB score in multitude time points were significantly higher in B group than in A group(P<0.05 or P<0.01). The number of apoptosis neurons in B group was less than in A group’s(P<0.05 or P<0.01). Conclusions:Insulin has neuroprotective effects on traumatic spinal cord injury in rat models. It can promote recovery of function by depressing apoptosis of neurons and promoting recovery and regeneration of neuraxon.

关键词

胰岛素脊髓损伤GAP-43凋亡BBB评分

Keywords

insulinspinal cord injuryGAP-43apoptosisBBB score

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