Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells

PENG Bo; et al

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Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells

更新时间：2024-01-17

- Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 551-554(2009)
- 作者机构：
  
  重庆医科大学附属第一医院泌尿外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： Q492
- Published：2009，
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[1]彭波,王德林.ATP50对生长激素诱导大鼠睾丸间质细胞睾酮分泌的影响[J].重庆医科大学学报,2009,34(05):551-554.

PENG Bo, et al. Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells. [J]. Journal of Chongqing Medical University 34(5):551-554(2009)
[1]彭波,王德林.ATP50对生长激素诱导大鼠睾丸间质细胞睾酮分泌的影响[J].重庆医科大学学报,2009,34(05):551-554. DOI：

PENG Bo, et al. Effect of ATP50 on GH-induced testosterone secretion in isolated rat Leydig cells. [J]. Journal of Chongqing Medical University 34(5):551-554(2009) DOI：

摘要

目的:研究ATP50在SD大鼠睾丸Leydig细胞中对生长激素（Growth hormone

GH）诱导睾酮合成及生长激素受体功能的影响。方法:采用离体细胞体外孵育法

观察反义ATP50寡脱氧核苷酸（Oligo deoxy nucletides

ODN）对GH诱导大鼠睾丸Leydig细胞分泌睾酮的影响及GH受体功能的影响

以睾酮放射免疫试剂盒测定睾酮合成的情况

cAMP放免试剂盒检测cAMP含量来评定GH受体的功能

免疫组织化学检测ATP50蛋白的表达。结果:反义ATP50 ODN呈剂量依赖性抑制GH诱导睾丸Leydig细胞睾酮的分泌（P<0.05）

同时使Leydig细胞中ATP50蛋白表达下降

而无义tat ODN则没有相应的作用

免疫组织化学显示ATP50蛋白阳性颗粒在Leydig细胞中中呈密集性分布

表达强度明显高于GH组（P<0.05）。结论:在SD大鼠睾丸Leydig细胞中

ATP50可能通过增强GH受体的功能

进而刺激GH诱导睾酮的合成。提示ATP50参与GH诱导大鼠睾丸Leydig细胞睾酮的分泌过程。

Abstract

Objective:Toinvestigate the effect of ATP50 on GH-induced testosterone secretion in isolated SD rat Leydigcells.Methods:The Leydig cells were treated with ATP50 antisense oligodeoxynucletides(Anti-ODN)in GH group

nonsense tat ODN in control group

cAMP and testosterone in culture medium was detected by radioimmunoassay

expression of ATP50 protein was investigated by immunohistochemistry.Results:Antisense ATP50 ODN significantly inhibited GH-induced testosterone secretion of isolated rat Leydig cells in a dose-dependent manner(P<0.05).At the same time

integral optical density immunostaining of ATP50 protein in Leydig cells was also remarkably reduced.Nonsense tat ODN had no effect on GH-induced testosterone secretion of Leydig cells

integral optical density immunostaining of ATP50 was remarkably more obvious in nonsense tat ODN group than in GH group(P<0.05).Conclusion:These results indicate that ATP50 might closely correlate with GH-reduced testosterone secretion.

关键词

ATP50间质细胞反义寡脱氧核苷酸睾酮

Keywords

ATP50Leydig cellsAnti-oligodeoxynucletides(anti-ODN)testosterone

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