Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines

GU Hai-tao; et al

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Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines

更新时间：2024-01-17

- Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 554-557(2009)
- 作者机构：
  
  重庆医科大学附属第二医院普外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2009，
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[1]顾海涛,王继见,李洋,杨成.siRNA干扰对乳腺癌(MCF-7)ATX表达和侵袭力的影响[J].重庆医科大学学报,2009,34(05):554-557.

GU Hai-tao, et al. Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines. [J]. Journal of Chongqing Medical University 34(5):554-557(2009)
[1]顾海涛,王继见,李洋,杨成.siRNA干扰对乳腺癌(MCF-7)ATX表达和侵袭力的影响[J].重庆医科大学学报,2009,34(05):554-557. DOI：

GU Hai-tao, et al. Effect of silencing ATX by RNA interference on expression and invasive potential of human breast cancer cell lines. [J]. Journal of Chongqing Medical University 34(5):554-557(2009) DOI：

摘要

目的:研究针对自分泌运动因子（Autotaxin

ATX）的小干扰RNA（Small interfering RNA

siRNA）对人乳腺癌细胞ATX基因表达的影响

探讨RNA干扰技术治疗乳腺癌的前景。方法:参考人黑色素瘤ENPP2基因序列

设计合成ATX-siRNA及随机阴性对照。在阳离子脂质体的介导下转染人乳腺癌细胞MCF-7

转染后48h收集细胞

用半定量RT-PCR和western blot检测转染后ATX mRNA和ATX蛋白的表达

体外实验测定肿瘤细胞穿透matrigel的能力。结果:体外合成的siRNA在转染乳腺癌细胞48h后ATX mRNA和蛋白表达降低

转染48h后测定乳腺癌MCF-7细胞的侵袭力明显降低（P<0.01）。结论:ATX-siRNA能有效抑制人乳腺癌细胞株ATX基因和蛋白的表达

降低人乳腺癌细胞的侵袭力。

Abstract

Objective:To study the effect of RNA interference(RNA)ion ATX-mRNA expression and the invasive petential of human breast cancer cell lines.Methods:Chemically synthesizeddouble stranded RNA(dsRNA)targeting ATX was transfected into human breast cancer cell MCF-7 using SiPORT Lipid.The transfection efficiency was observed under fluorescence confocal microscope.Expression of ATX mRNA and protein was detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Cell penetrate matrigel capacity were determined by in vitro experiment.Results:ATX-siRNA effectively inhibited ATX-mRNA and protein expression(P<0.05)

and degraded invasion capability of MCF-7 cells(P<0.01).Conclusion:ATX-siRNA inhibits ATX-mRNA and protein expression

and degrades invasion of human breast cancer cell.

关键词

小干扰RNA人乳腺癌细胞MCF-7自分泌运动因子乳腺肿瘤

Keywords

small interfering RNA(siRNA)MCF-7Autotoxin(ATX)Breast cancer

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Related Institution

Department of Pathology,College of Basic Medicine,Chongqing Medical University

重庆医科大学病理学教研室重庆400016 常州市肿瘤医院病理科

Department of General Surgery,the First Affiliated Hospital,Chongqing Medical University

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