Protective effect of Shenfu injection on myocardial energy metabolism in septic mice

LIU Qiong; et al

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Protective effect of Shenfu injection on myocardial energy metabolism in septic mice

更新时间：2024-01-17

- Protective effect of Shenfu injection on myocardial energy metabolism in septic mice
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 547-550(2009)
- 作者机构：
  
  1. 重庆医科大学附属第一医院中心ICU
  2. 重庆医科大学基础医学院病理学教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R285.5
- Published：2009，
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[1]刘琼,周发春,徐昉,曹友德.参附注射液对腹腔感染脓毒症小鼠心肌能量代谢的保护作用研究[J].重庆医科大学学报,2009,34(05):547-550.

LIU Qiong, et al. Protective effect of Shenfu injection on myocardial energy metabolism in septic mice. [J]. Journal of Chongqing Medical University 34(5):547-550(2009)
[1]刘琼,周发春,徐昉,曹友德.参附注射液对腹腔感染脓毒症小鼠心肌能量代谢的保护作用研究[J].重庆医科大学学报,2009,34(05):547-550. DOI：

LIU Qiong, et al. Protective effect of Shenfu injection on myocardial energy metabolism in septic mice. [J]. Journal of Chongqing Medical University 34(5):547-550(2009) DOI：

摘要

目的:观察腹腔感染脓毒症小鼠心肌能量代谢情况

用参附注射液进行干预治疗

探讨参附注射液对其能量代谢的保护作用。方法:采用盲肠结扎穿孔法（Cecal ligation and puncture

CLP）复制腹腔感染脓毒症动物模型。83只小鼠随机分为CLP组（感染组）、CLP加参附注射液治疗组（治疗组）和假CLP组（对照组）。于造模后6h、12h及24h分别采用电镜和形态计量学方法观察心肌线粒体形态、数量和膜磷脂定位;酶细胞化学法分析线粒体细胞色素氧化酶（Cytochrome oxidase

CCO）和琥珀酸脱氢酶（Succinate dehydrogenase

SDH）活性

反相高效液相色谱法测定心肌组织ATP、ADP和AMP含量。结果:感染组小鼠心肌线粒体大量破坏

膜磷脂严重缺失、定位改变;CCO和SDH活性降低

ATP、ADP和AMP含量下降。各时间点治疗组上述各项指标均较感染组显著改善。结论:脓毒症小鼠心肌线粒体结构严重破坏、功能明显下降

参附注射液能有效保护脓毒症小鼠心肌线粒体结构和功能

改善心肌能量代谢。

Abstract

Objective:To investigate the changes of myocardial energy production in septic mice after a cecal ligation and puncture

and to observe the intervention effects of Shenfu injection.Methods:Mice models of abdominal infection were established by a cecal ligation and puncture(CLP).Eighty-three mice were randomly divided into three groups:CLP group(infection group)

CLP and treated with Shenfu injection group(treatment group)and pseudooperation control group.The morphology and membrane phospholipid of myocardial mitochondria were studied by using transmission electronmicroscope morphometry method.The activities of cytochrome oxidase(CCO)and succinate dehydrogenase(SDH)of myocardial mitochondria were also detected by enzyme cytochemical method.Reversed phase high-performance liquid chromatography(RP-HPLC)was used to analyze contents of myocardial ATP

ADP and AMP.Results:As compared to control group

a large number of mitochondria were damaged in infection group

the apparent mitochondrial membrane phospholipid deletion and localization change were observed

the activities of CCO and SDH in mitochondria declined obviously

and the contents of ATP

ADP as well as AMP declined notably.All of these were obviously improved in treatment group.Conclusion:The myocardial mitochondrial structure destroyed and its functions declined apparently in septic mice.Shenfu injection could protect the myocardial mitochondrial structure and function effectively

improve the myocardial energy metabolism in septic mice also.

关键词

腹腔感染脓毒症心肌线粒体能量代谢参附注射液

Keywords

Abdominal infectionsepsisMyocardial mitochondriaenergy metabolismShenfu injection

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