Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line

MA Jie; et al

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Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line

更新时间：2024-01-17

- Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line
- Journal of Chongqing Medical University Vol. 34, Issue 5, Pages: 542-546(2009)
- 作者机构：
  
  重庆医科大学附属第二医院普外科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R737.9
- Published：2009，
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[1]马杰,王继见,李洋.siRNA沉默FAK基因表达对乳腺癌MCF-7细胞生物学特性的影响[J].重庆医科大学学报,2009,34(05):542-546.

MA Jie, et al. Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line. [J]. Journal of Chongqing Medical University 34(5):542-546(2009)
[1]马杰,王继见,李洋.siRNA沉默FAK基因表达对乳腺癌MCF-7细胞生物学特性的影响[J].重庆医科大学学报,2009,34(05):542-546. DOI：

MA Jie, et al. Effect of silencing FAK gene expression by siRNA on characteristics of human breast cancer MCF-7 cell line. [J]. Journal of Chongqing Medical University 34(5):542-546(2009) DOI：

摘要

目的:研究RNAi干扰粘着斑激酶（Focal adhesion kinase

FAK）的表达及对人类乳腺癌细胞生物学特性的影响。方法:构建表达FAK基因siRNA的重组质粒FAK-siRNA

在脂质体的介导下转染入人乳腺癌MCF-7细胞

荧光倒置显微镜下观察转染效率

应用RT-PCR和Westernblot检测FAKmRNA和蛋白表达

流式细胞术分析细胞周期分布

体外实验测定肿瘤细胞穿透matrigel的能力。结果:成功转染FAK-siRNA后乳腺癌MCF-7细胞FAK基因的表达与阴性对照组、空白质粒转染组相比FAKmRNA和蛋白表达水平明显降低（P<0.05）

细胞增殖指数及侵袭力明显降低（P<0.01）。结论:FAK-siRNA能有效抑制人乳腺癌细胞FAK基因mRNA和蛋白的表达

降低细胞增殖能力及侵袭力。

Abstract

Objective:To study the effect ofRNA interferenc(RNA)ion FAK gene expression and the biological characteristics of human breast cancer MCF-7.Methods:The constructed recombinant plasmid FAK-siRNA was transfected into human breast cancer MCF-7 cell using LipofectamineTM 2000.The transfection efficiency was observed under fluorescence confocal microscope.Expression of FAK mRNA and protein were detected by reverse transcription polymerase chain reaction(RT-PCR)and Western blot.Distribution of cell cycle was assessed by flow cytometry.Cell penetrate matrigel capacity were determined by invitro experiment.Results:FAK-siRNA effectively inhibited FAK mRNA and protein expression(P<0.05).The proliferation index and the invasion capability of MCF-7 cells were effectively degraded(P<0.01).Conclusion:FAK-siRNA inhibits the expression of FAK mRNA and protein

degrades the proliferation and the invasion capability of human breast cancer cell.

关键词

RNA干扰乳腺癌粘着斑激酶增殖侵袭

Keywords

RNA interferencebreast cancerFocal adhesion kinaseproliferationTumor invasion

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Department of Pathophysiology,College of Basic Medicine,Chongqing Medical University

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