Objective:To establish an HPLC method for the determination of the concentration of ganciclovir in vitro

and to inspect sustain-release of medical biofibrin glue carrying Ganciclovir. Methods:The HPLC system consisted of water-methanol(98:2)as mobile phase with the flowrate of 1.0 ml/min

and the detection wavelength was 252 nm and the injection volumn was 20 μl. Column tempetature was 30℃. Results:The linear range of ganciclovir was 5~350 μg/ml(r=0.9996)

displaying a good linear relationship. The within-day and between-days relative standard deviations(RSD)were 0.89%～2.35%. The methodological recovery ranged from 98.4 % to 100.8 %. The elaborated method was sensitive

precise and applicable for determining the concentration and release rate of ganciclovir in vitro. Conclusion:The sustain-release effect of medical biofibrin glue carrying ganciclovir in vitro is good.