Preparation of doxorubicin liposome and their effect on human MCF-7

YAN Ke; et al

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Preparation of doxorubicin liposome and their effect on human MCF-7

更新时间：2024-01-17

- Preparation of doxorubicin liposome and their effect on human MCF-7
- Journal of Chongqing Medical University Vol. 34, Issue 3, Pages: 329-331(2009)
- 作者机构：
  
  重庆医科大学药学院药化教研室
- 作者简介：
- 基金信息：
- DOI：
  CLC： R96;R943
- Published：2009，
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[1]颜科,胡春梅,潘黎军,王驰.阿霉素脂质体的制备及对MCF-7/DOX作用的考察[J].重庆医科大学学报,2009,34(03):329-331.

YAN Ke, et al. Preparation of doxorubicin liposome and their effect on human MCF-7. [J]. Journal of Chongqing Medical University 34(3):329-331(2009)
[1]颜科,胡春梅,潘黎军,王驰.阿霉素脂质体的制备及对MCF-7/DOX作用的考察[J].重庆医科大学学报,2009,34(03):329-331. DOI：

YAN Ke, et al. Preparation of doxorubicin liposome and their effect on human MCF-7. [J]. Journal of Chongqing Medical University 34(3):329-331(2009) DOI：

摘要

目的:制备阿霉素（Doxorubicin

DOX）脂质体

并考察其对耐阿霉素的人乳腺癌细胞（MCF-7/DOX）的作用。方法:采用硫酸铵梯度法制备脂质体;采用MTT法考察DOX脂质体对MCF-7/DOX的细胞毒性和逆转多药耐药（Multydrug resistance

MDR）的效果。结果:阿霉素的平均包封率为90.77%。脂质体的细胞毒性实验结果显示

DOX脂质体的IC50比游离DOX小4倍;DOX脂质体对MCF-7/DOX的逆转倍数是游离DOX的4倍。结论:硫酸铵梯度法适用于制备DOX脂质体

其具有较好的细胞毒性作用及一定的逆转MCF-7/DOXMDR的作用。

Abstract

Objective:To prepare doxorubicin liposome and investigate its effect on human MCF-7 in vitro. Methods:DOX liposome was prepared by(NH4)2SO4-gradient method.The MTT method was used to test cytotoxicity and reverse multidrug resistance of DOX liposome on MCF-7/DOX. Results:DOX was encapsulated by 90.77%. The results of liposome MTT experiment suggested that IC50 of free DOX was 4 times bigger than DOX liposome.The DOX liposomes’reversing effect on MDR was 4 times as much as that of free DOX. Conclusion:The method of(NH4)2SO4 gradient is suitable for producing DOX liposome and it has better cytotoxicity and reversing MDR effect on MCF-7 than free DOX.

关键词

阿霉素脂质体硫酸铵梯度多药耐药人乳腺癌细胞

Keywords

DOXliposome(NH4)2SO4gradientmethodMultidrugresistanceMCF-7

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