Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor

LI Wen-ying; et al

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Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor

更新时间：2024-01-17

- Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor
- Journal of Chongqing Medical University Vol. 34, Issue 4, Pages: 424-427(2009)
- 作者机构：
  
  重庆医科大学附属口腔医院正畸科
- 作者简介：
- 基金信息：
- DOI：
  CLC： R392
- Published：2009，
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[1]李闻颖,邓锋,王豫蓉,陈允嘉,胡辉.不同浓度粒巨噬细胞集落刺激因子在未成熟树突状细胞培养中的效果研究[J].重庆医科大学学报,2009,34(04):424-427.

LI Wen-ying, et al. Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor. [J]. Journal of Chongqing Medical University 34(4):424-427(2009)
[1]李闻颖,邓锋,王豫蓉,陈允嘉,胡辉.不同浓度粒巨噬细胞集落刺激因子在未成熟树突状细胞培养中的效果研究[J].重庆医科大学学报,2009,34(04):424-427. DOI：

LI Wen-ying, et al. Generation of immature dendritic cells with different dose of granulocyte-macrophage colony-stimulating factor. [J]. Journal of Chongqing Medical University 34(4):424-427(2009) DOI：

摘要

目的:探讨不同浓度细胞因子在培养小鼠骨髓来源未成熟树突状细胞（Dendriticcells

DC）中的作用

寻找培养所需最佳细胞因子浓度。方法:分别用低、中、高剂量粒细胞-巨噬细胞集落刺激因子（Granulocyte-macrophagecolony-stimulatingfactor

GM-CSF）联合或不联合白细胞介素4（Interlenkin-4

IL-4）培养小鼠骨髓源性未成熟DC

对其进行形态学观察和细胞表型检测。结果:培养第7d

低剂量组DC数量最少、突起细小

CD80、CD86及主要组织相容（Major histo compatibilitycomplex

MHCⅡ）类分子表达低

如联合IL-4培养可提高DC数量;中等剂量组与高剂量组DC较低剂量组为多

CD80、CD86及MHCⅡ类分子表达亦较前者提高

但二组无明显差异

有无联合IL-4培养的差异亦不显著。结论:GM-CSF低剂量时

可诱导出纯度较高的未成熟DC

而GM-CSF中等剂量和高剂量时

无论是否联合诱导IL-4培养

诱导未成熟DC的产量及纯度无明显差异。

Abstract

Objective:To demonstrate the effect of cytokine with different concentration in mouse bone marrow-derived immature DCs and find the optimal cytokine dose for DC culture.Methods:mouse bone marrow-derived immature DCs were cultured in the presence of low

moderate and high dose of GM-CSF cytokine with or without IL-4.The morphology of cells were observed and the phenotype were detected.Results:On day 7

DCs in low dose group had the least quantity

small cell process and low expression of CD80

CD86 and MHC class II.The quantity of DCs increased if cultured with IL-4;DCs in moderate dose group and high dose group were more than that in low dose group and the expression of CD80

CD86 and MHC classⅡwere higher

but it is no difference between moderate dose group and high dose group

however cultured with or without IL-4.Conclusion:Higher purity of immature DCs could be get when cultured with low dose of GM-CSF.It was no difference of yield and purity of immature DCs when cultured with moderate or high dose of GM-CSF whether or not combine IL-4.

关键词

树突状细胞粒细胞-巨噬细胞集落刺激因子白细胞介素-4

Keywords

dendritic cellsGranulocyte-macrophage colony-stimulatingfactorinterleukin-4

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